Prevalence and molecular characterization of Cryptosporidium spp. and Giardia intestinalisin household dogs and catsfrom Shiraz, Southwestern Iran


G. intestinalis

How to Cite

Homayouni, M. M., Razavi, S. M., shaddel, M., & Asadpour, M. (2020). Prevalence and molecular characterization of Cryptosporidium spp. and Giardia intestinalisin household dogs and catsfrom Shiraz, Southwestern Iran. Veterinaria Italiana, 55(4), 311-318.


In the present study, a total of 615 fecal samples from veterinary clinics were screened by microscopy for the presence of Cryptosporidium and Giardia oocysts. Molecular genotyping of Cryptosporidium and Giardia were carried out using PCR and sequence analysis. Overall, Cryptosporidium and Giardia oocysts were detected in the 0.6% (2/315) and 1.9% (6/315) of dogs and in the 0.7% (2/300) and 1.3% (4/300) of cats, respectively. Sequencing revealed the presence of C. canis (n = 2) in dogs and C. felis (n = 2) in cats. Moreover, G. intestinalisassemblage D (n = 2), C (n = 3) and A, sub-assemblage AII (n = 1) were identified in dogs; G. intestinalis assemblage F (n = 3) and assemblage A, sub-assemblage AI (n = 1) were identified in cats. The highest prevalence of Giardia was observed in dogs younger than one year (6/315), and in those with diarrhea (p < 0.05). Data of the study suggest that dogs and cats play a minor role in the zoonotic transmission of cryptosporidiosis and giardiasis in Southwestern Iran.


Asadpour M., Namazi F., Razavi S.M. & Nazifi S. 2018. Comparative efficacy of curcumin and paromomycin against Cryptosporidium parvum infection in a BALB/c model. Vet Parasitol, 250, 7-14.

Bahrami A., Doosti, A., Nahravanian H., Noorian A.M. & Asbchin S.A. 2011. Epidemiological survey of gastro-intestinal parasites in stray dogs and cats. Aust J Basic Appl Sci, 5, 1944-1948.

Beiromvand M., Akhlaghi L., Massom S.H.F., Meamar A.R., Motevalian A., Oormazdi H. & Razmjou E. 2013. Prevalence of zoonotic intestinal parasites in domestic and stray dogs in a rural area of Iran. Prev Vet Med, 109, 162-167.

Berahmat R., Spotin A., Ahmadpour E., Mahami-Oskouei M., Rezamand A., Aminisani N., Ghojazadeh M., Ghoyounchi R. & Mikaeili-Galeh T. 2017. Human cryptosporidiosis in Iran: a systematic review and meta-analysis. Parasitol Res, 116, 1111-1128.

Berrilli F., D’Alfonso R., Giangaspero A., Marangi M., Brandonisio O., Kaboré Y., Glé C., Cianfanelli C., Lauro R. & Di Cave D. 2012. Giardia duodenalis genotypes and Cryptosporidium species in humans and domestic animals in Côte d’Ivoire: occurrence and evidence for environmental contamination. Trans R Soc Trop Med Hyg, 106, 191-195.

Bowman D.D. & Lucio-Forster A. 2010. Cryptosporidiosis and giardiasis in dogs and cats: veterinary and public health importance. Exp Parasitol, 124, 121-127.

Cacciò S.M., De Giacomo M. & Pozio E. 2002. Sequence analysis of the β-giardin gene and development of a polymerase chain reaction–restriction fragment length polymorphism assay to genotype Giardia duodenalis cyst from human faecal samples. Int J Parasitol, 32, 1023-1030.

Cacciò S.M. & Ryan U. 2008. Molecular epidemiology of giardiasis. Mol Biochem Parasitol, 160, 75-80.

Cacciò S.M., Thompson R.A., McLauchlin J. & Smith H.V. 2005. Unravelling Cryptosporidium and Giardia epidemiology. Trends Parasitol, 21, 430-437.

De Lucio A., Bailo, B., Aguilera, M., Cardona, G.A., Fernández-Crespo, J.C. & Carmena, D. 2017. No molecular epidemiological evidence supporting household transmission of zoonotic Giardia duodenalis and Cryptosporidium spp. from pet dogs and cats in the province of Álava, Northern Spain. Acta Trop, 170, 48-56.

Efstratiou A., Ongerth, J.E. & Karanis, P. 2017. Waterborne transmission of protozoan parasites: Review of worldwide outbreaks-An update 2011–2016. Water Res, 114, 14-22.

Hooshyar H., Ghafarinasab S., Arbabi M., Delavari M. & Rasti S. 2017. Genetic variation of Giardia lamblia isolates from food-handlers in Kashan, Central Iran. Iran J Parasitol , 12, 83.

Hopkins R.M., Meloni B.P., Groth D.M., Wetherall J.D., Reynoldson J.A. & Thompson R.A. 1997. Ribosomal RNA sequencing reveals differences between the genotypes of Giardia isolates recovered from humans and dogs living in the same locality. J Parasitol, 44-51.

Itoh N., Muraoka N., Kawamata J., Aoki M. & Itagaki T. 2006. Prevalence of Giardia intestinalis infection in household cats of Tohoku district in Japan. J Vet Med Sci, 68, 161-163.

Itoh N., Oohashi Y., Ichikawa-Seki M., Itagaki T., Ito Y., Saeki H., Kanai K., Chikazawa S., Hori Y. & Hoshi F. 2014. Molecular detection and characterization of Cryptosporidium species in household dogs, pet shop puppies, and dogs kept in a school of veterinary nursing in Japan. Vet Parasitol, 200, 284-288.

Kasaei R., Carmena, D., Jelowdar, A. & Beiromvand, M. 2018. Molecular genotyping of Giardia duodenalis in children from Behbahan, southwestern Iran. Parasitol Res, 117, 1425-1431.

Karim M.R., Zhang S., Jian F., Li J., Zhou C., Zhang L., Sun M., Yang G., Zou F. & Dong H. 2014. Multilocus typing of Cryptosporidium spp. and Giardia duodenalis from non-human primates in China. Int J Parasitol, 44, 1039-1047.

Khademvatan S., Rahman Abdizadeh F.R., Hashemitabar M., Ghasemi M. & Tavalla M. 2014. Stray cats gastrointestinal parasites and its association with public health in Ahvaz City, South Western of Iran. Jundishapur J Microbiol, 7,8.

Kohansal M.H., Fazaeli A., Nourian, A., Haniloo, A. & Kamali, K. 2017. Dogs’ gastrointestinal parasites and their association with public health in Iran. J Vet Res, 61, 189-195.

Lagapa J.T.G., Oku Y., Kaneko M., Ganzorig S., Ono T., Nonaka N., Kobayashi F. & Kamiya M. 2009. Monitoring of environmental contamination by Echinococcus multilocularis in an urban fringe forest park in Hokkaido, Japan. Environ Health Prev Med, 14, 299.

Lucio-Forster A., Griffiths J.K., Cama V.A., Xiao L. & Bowman D.D. 2010. Minimal zoonotic risk of cryptosporidiosis from pet dogs and cats. Trends Parasitol, 26, 174-179.

Mirzaghavami M., Sadraei J. & Forouzandeh M. 2016. Detection of Cryptosporidium spp. in free ranging animals of Tehran, Iran. J Parasit Dis, 40, 1528-1531.

Neves D., Lobo L., Simões P.B. & Cardoso L. 2014. Frequency of intestinal parasites in pet dogs from an urban area (Greater Oporto, northern Portugal). Vet Parasitol, 200, 295-298.

Pallant L., Barutzki D., Schaper R. & Thompson R.A. 2015. The epidemiology of infections with Giardia species and genotypes in well cared for dogs and cats in Germany. Parasites & vectors, 8, 2.

Pavlasek I. & Ryan U. 2007. The first finding of a natural infection of Cryptosporidium muris in a cat. Vet Parasitol, 144, 349-352.

Ranjbar R., Mirhendi H., Izadi M., Behrouz B. & Mohammadi Manesh R. 2018. Molecular Identification of Cryptosporidium spp. in Iranian Dogs Using Seminested PCR: A First Report. Vector Borne Zoonotic Dis, 18, 96-100.

Rehbein S., Klotz C., Ignatius R., Müller E., Aebischer A. & Kohn B. 2019. Giardia duodenalis in small animals and their owners in Germany: A pilot study. Zoonoses Public Health, 66, 117-124.

Ryan U. & Cacciò S.M. 2013. Zoonotic potential of Giardia. Int J Parasitol, 43, 943-956,

Santín M., Trout J.M., Vecino J.A.C., Dubey J. & Fayer R. 2006. Cryptosporidium, Giardia and Enterocytozoon bieneusi in cats from Bogota (Colombia) and genotyping of isolates. Vet Parasitol, 141, 334-339.

Shoorijeh S.J., Sadjjadi S.M., Asheri A. & Eraghi K. 2008. Giardia spp. and Sarcocystis spp. status in pet dogs of Shiraz, Southern part of Iran. Trop Biomed, 25, 154-159.

Sotiriadou I., Pantchev N., Gassmann D. & Karanis P. 2013. Molecular identification of Giardia and Cryptosporidium from dogs and cats. Parasite, 20,

Taghipour N., Nazemalhosseini-Mojarad E., Haghighi A., Rostami-Nejad M., Romani S., Keshavarz A., Alebouyeh M. & Zali M. 2011. Molecular epidemiology of cryptosporidiosis in Iranian children, Tehran, Iran. Iran J Parasitol, 6, 41.

Tamura K., Stecher G., Peterson D., Filipski A. & Kumar S. 2013. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol, 30, 2725-2729.

Uehlinger F.D., Greenwood S.J., McClure J.T., Conboy G., O’Handley R. & Barkema H.W. 2013. Zoonotic potential of Giardia duodenalis and Cryptosporidium spp. and prevalence of intestinal parasites in young dogs from different populations on Prince Edward Island, Canada. Vet Parasitol, 196, 509-514.

Xiao L. 2010. Molecular epidemiology of cryptosporidiosis: an update. Exp Parasitol, 124, 80-89.

Xiao L., Alderisio K. & Singh A. 2006. Development and standardization of a Cryptosporidium genotyping tool for water samples, J Am Water Works Assoc. 5-9.

Yang R., Ying J.L.J., Monis P. & Ryan U. 2015. Molecular characterisation of Cryptosporidium and Giardia in cats (Felis catus) in Western Australia. Exp Parasitol, 155, 13-18.