A case report of sporadic ovine listerial meningoencephalitis in Kosovo
DOI:
https://doi.org/10.12834/2166.12781.3Keywords:
Antimicrobial susceptibility, Brain, MIC, Ovine listeriosis, SerotypingAbstract
In 2018, a case of neural disease suspected of listeriosis was reported in a flock of sheep in Kosovo with the death of ewes and 5 lambs. Samples from the brain of only three dead animals were subjected to histopathological and bacteriological analysis. MALDI-TOF MS was applied to confirm suspected Listeria spp. isolates from culture and multiplex PCR was applied for molecular serotyping. All isolates were tested for antimicrobial susceptibility by microdilution broth method. The histopathological analysis of the brain specimens showed typical changes for Listeria monocytogenes. Listeria spp. was isolated in brain samples from all three animals, and all the isolates were confirmed using MALDI-TOF MS and PCR down to the species level (Listeria monocytogenes). The molecular characterisation using multiplex PCR revealed all isolates as Listeria monocytogenes serotype 4b. All L. monocytogenes isolates were found to be susceptible to penicillin, erythromycin, tetracycline, streptomycin, trimethoprim/sulfamethosazole, quinupristin/dalfopristin, kanamycin, vancomycin, and gentamicin but resistant to nitrofurantoin and lincomycin. This study shows the emergence of a highly virulent strain in sheep farms in Kosovo and a possible threat to public health.
References
Amato E., Filipello V., Gori M., Lomonaco S., Losio M.N., Parisi A., Huedo P., Knabel S.J. & Pontello M. 2017 Identification of a major Listeria monocytogenes outbreak clone linked to soft cheese in Northern Italy-2009-2011. Bmc Infectious Diseases, 17 (1):342.
Bartt R. 2000. Listeria and atypical presentations of Listeria in the central nervous system. Semin Neurol, 20 (30), 361-373.
Bertsch D., Rau J., Eugster M.R., Haug M.C., Lawson P.A., Lacroix C. & Meile L. 2013. Listeria fleischmannii sp. nov., isolated from cheese. Int J Syst Evol Microbiol, 63 (Pt 2), 526-532.
Brugere-Picoux J. 2008. Ovine listeriosis. Small Ruminant Res, 76 (1-2), 12-20.
Buchanan R.L., Gorris L.G.M., Hayman M.M., Jackson T.C. & Whiting R.C. 2017. A review of Listeria monocytogenes: An update on outbreaks, virulence, dose-response, ecology, and risk assessments. Food Control, 75, 1-13.
Clinical and Laboratory Standards Institute (CLSI). 2014. Performance standards for antimicrobial susceptibility testing; Twenty-fourth informational supplement. 2014. M100 S24:, wayne (PA).
Conter M., Paludi D., D’Orio V., Vergara A. & Ianieri A. 2007. Antimicrobial susceptibility of Listeria monocytogenes isolated from food and food-processing environment. Ann Fac Medic Vet di Parma. XXVII, 157–164.
Dons L., Jin Y., Kristensson K. & Rottenberg M.E. 2007. Axonal transport of Listeria monocytogenes and nerve-cell-induced bacterial killing. J Neurosci Res, 85 (12), 2529-2537.
Doumith M., Buchrieser C., Glaser P., Jacquet C. & Martin P. 2004. Differentiation of the major Listeria monocytogenes serovars by multiplex PCR. J Clin Microbiol, 42 (8), 3819-3822.
George L.W. 2002. Listeriosis. In: Large Animal Internal Medicine. edn. Edited by Smith BP. St. Louis, MO: Mosby.
Giannati-Stefanou A., Tsakos P., Bourtzi-Hatzopoulou E. & Anatoliotis K. 2006. Study of microbiological aspects of meningoencephalitis due to Listeria spp in ruminants. Journal of the Hellenic Veterinary Medical Society, 57 (4) : 275-288.
Godreuil S., Galimand M., Gerbaud G., Jacquet C. & Courvalin P. 2003. Efflux pump Lde is associated with fluoroquinolone resistance in Listeria monocytogenes. Antimicrob Agents Chemother, 47 (2), 704-708.
Granier S.A., Moubareck C., Colaneri C., Lemire A., Roussel S., Dao T.T., Courvalin P. & Brisabois A. 2011. Antimicrobial Resistance of Listeria monocytogenes Isolates from Food and the Environment in France over a 10-year period. Appl Environ Microb, 77 (8), 2788-2790.
Heger W., Dierich M.P. & Allerberger F. 1997. In vitro susceptibility of Listeria monocytogenes : comparison of the E test with the agar dilution test. Chemotherapy, 43 (5), 303-310.
Henke D., Rupp S., Gaschen V., Stoffel M.H., Frey J., Vandevelde M. & Oevermann A. 2015. Listeria monocytogenes spreads within the brain by actin-based intra-axonal migration. Infect Immun, 83 (6), 2409-2419.
Kerouanton A., Marault M., Petit L., Grout J., Dao T.T. & Brisabois A. 2010. Evaluation of a multiplex PCR assay as an alternative method for Listeria monocytogenes serotyping. J Microbiol Methods, 80 (2), 134-137.
Kotzamanidis C., Papadopoulos T., Vafeas G., Tsakos P., Giantzi V. & Zdragas A. 2019. Characterization of Listeria monocytogenes from encephalitis cases of small ruminants from different geographical regions, in Greece. J Appl Microbiol, 126 (5), 1373-1382.
Kumar H., Singh B.B., Bal M.S., Kaur K., Singh R., Sidhu P.K. & Sandhu K.S. 2007. Pathological and epidemiological investigations into listerial encephalitis in sheep. Small Ruminant Res, 71 (1-3), 293-297.
Lozano C., Aspiroz C., Ara M., Gomez-Sanz E., Zarazaga M. & Torres C. 2011. Methicillin-resistant Staphylococcus aureus (MRSA) ST398 in a farmer with skin lesions and in pigs of his farm: clonal relationship and detection of Inu(A) gene. Clin Microbiol Infect, 17 (6), 923-927.
Low J.C., Wright F., Mclauchlin J. & Donachie W. 1993. Serotyping and Distribution of Listeria Isolates from Cases of Ovine Listeriosis. Veterinary record, 133 (7), 165-166.
Lyon S.A., Berrang M.E., Fedorka-Cray P.J., Fletcher D.L. & Meinersmann R.J. 2008. Antimicrobial resistance of Listeria monocytogenes isolated from a poultry further processing plant. Foodborne Pathog Dis, 5 (3), 253-259.
Maxie M.G. & Youssef S. 2007. Nervous system. In: Maxie MG, editor. Jubb, Kennedy, and Palmer’s Pathology of domestic animals. Vol. 1. Philadelphia: Saunders/Elsevier; pp. 405–408.
Nash M.L., Hungerford L.L., Nash T.G. & Zinn G.M. 1995. Epidemiology and Economics of Clinical Listeriosis in a Sheep Flock. Prev Vet Med, 24 (3), 147-156.
Nightingale K.K., Schukken Y.H., Nightingale C.R., Fortes E.D., Ho A.J., Her Z., Grohn Y.T., McDonough P.L. & Wiedmann M. 2004. Ecology and transmission of Listeria monocytogenes infecting ruminants and in the farm environment. Appl Environ Microb, 70 (8), 4458-4467.
Noll M., Kleta S. & Al Dahouk S. 2018. Antibiotic susceptibility of 259 Listeria monocytogenes strains isolated from food, food-processing plants and human samples in Germany. J Infect Public Health, 11 (4), 572-577.
Rocha P.R.D., Lomonaco S., Bottero M.T., Dalmasso A., Dondo A., Grattarola C., Zuccon F., Iulini B., Knabel S.J. & Capucchio M.T. 2013. Ruminant Rhombencephalitis-Associated Listeria monocytogenes Strains Constitute a Genetically Homogeneous Group Related to Human Outbreak Strains (vol 79, pg 3059, 2013). Appl Environ Microb, 79(22), 7114-7114.
Spanjaard L. & Vandenbroucke-Grauls C.M. 2008. Activity of daptomycin against Listeria monocytogenes isolates from cerebrospinal fluid. Antimicrob Agents Chemother, 52(5), 1850-1851.
Vela A.I., Fernandez-Garayzabal J.F., Latre M.V., Rodriguez A.A., Dominguez L. & Moreno M.A. 2001. Antimicrobial susceptibility of Listeria monocytogenes isolated from meningoencephalitis in sheep. Int J Antimicrob Agents, 17(3), 215-220.
Wesley I.V., Larson D.J., Harmon K.M., Luchansky J.B & Schwartz A.R. 2002. A case report of sporadic ovine listerial meningoencephalitis in Iowa with an overview of livestock and human cases. Journal of veterinary Diagnostic Investigation, 14(4), 314-321.
World Health Organisation (WHO). 2016. Critically important antimicrobials for human medicine. In., 5th Revision edn. Geneva, Switzerland.
Wiedmann M., Czajka J., Bsat N., Bodis M., Smith M.C., Divers T.J. & Batt C.A. 1994. Diagnosis and epidemiological association of Listeria monocytogenes strains in two outbreaks of listerial encephalitis in small ruminants. J Clin Microbiol, 32(4), 991-996.
Zachary J.F.2012. Listeriosis. In: Zachary JF, McGavin MD, editors. Pathologic basis of veterinary disease. St Louis, Missouri: Elsevier/Mosby. pp. 192–195.
