1
Isolation and bioinformatics analysis of the NADC30_Like CJS01 strain of the porcine reproductive and respiratory syndrome virus
VetIt.2182.14564.1

Keywords

Bioinformatics analysis
Isolation
PRRSV NADC30‑Like

How to Cite

TU, T., WANG, Y., LIAO, C. Y., ZHANG, P. F., XIANG, M. Y., YANG, Z. X., YAO, X. P., & LUO, Y. (2022). Isolation and bioinformatics analysis of the NADC30_Like CJS01 strain of the porcine reproductive and respiratory syndrome virus. Veterinaria Italiana, 58(1), 47–55. https://doi.org/10.12834/VetIt.2182.14564.1

Abstract

In this study, lung tissue was collected from nursery piglets suspected of being infected with porcine reproductive and respiratory syndrome virus (PRRSV) in a large‑scale pig farm in Sichuan, China. Polymerase chain reaction (PCR) and reverse transcription quantitative‑polymerase chain reaction (RT‑qPCR) methods were used to ensure that no other pathogens were present. Virus isolation was also carried out where the presence of PRRSV was determined by indirect immunoinfluscent assay (IFA). Compared with the common PRRSV strain, the isolate did not produce evident Cytopathic effect (CPE) in the early stage of isolation. CPE was found in the late stage, and the titer was 104.17 TCID50/0.1 mL. The strain was named CJS01. Bioinformatics analysis showed that it was a NADC30‑Like strain. The virus load was determined by measuring the nucleic acid load during the proliferation of the strain on Marc‑145 cells. The strain showed good adaptability on cells, and the virus proliferated on cells for 84 hr when the highest nucleic acid load was achieved. By recombinant analysis of ORF3~7 genes and prediction of its epitope, it was found that CJS01 strain might interfere with the immunesystem of the infected animals.
https://doi.org/10.12834/VetIt.2182.14564.1
VetIt.2182.14564.1

References

Alemán F. , Nieves Cordones M., Martínez V. & Rubio F. 2009. Differential regulation of the genes encoding the high-affinity K + transporters HAK5 of Thellungiella halophila and Arabidopsis thaliana in response to salinity. Environmental Experimental Botany, 65, 263-269.

Baltag G. & Baranov E. 2013. Characteristics and strategic development of pork sector in Moldova and the European Union. Management, Economic Engineering in Agriculture and Rural Development Scientific Papers Series,13 (4), 45-48. http://managementjournal.usamv.ro/pdf/vol3_4/Art7.pdf

Chen, Y. F., ZHAO, Y. R.& ZHAO, J. Z. 2008. PRRSV infectious clone and its application in vaccine research.C. Symposium on Prevention and Control of Pig Diseases, Animal Infectious Diseases Branch, Chinese Society of Animal Husbandry and Veterinary Medicine.

Dee, S., Otake, S. & Oliveira, S. 2012. Confirmation of the hypothesis of long-range airborne transmission of porcine reproductive and respiratory syndrome virus and mycoplasma hyopneumoniae. J. Prrspective.

Done S.H., Paton D.J. & White M.E. 1996. Porcine reproductive and respiratory syndrome (PRRS): a review, with emphasis on pathological, virological and diagnostic aspects. Br Vet J, 152(2), 153-174. https://doi.org/10.1016/s0007-1935(96)80071-6

Duan E.Z. 2011. [Studies on the difference of PRRSV infectious and PRRSV ADE]. Master Thesis. Henan Agricultural University.

Forsberg R., Storgaard T., Nielsen H. S., Oleksiewicz M. B., Cordioli P., Sala G., Hein J. & Bøtner A. 2002. The genetic diversity of European type PRRSV is similar to that of the North American type but is geographically skewed within Europe. Virology, 299(1), 38–47. https://doi.org/10.1006/viro.2002.1450

Gen H.D., Xu Y., Xie L.H., Lu Y. & Zhang H. 2012. [Research Progress in Porcine Reproductive and Respiratory Syndrome Virus Immunobiology]. Chinese Journal of Wildlife, 33,236-245.

Giles J. C., Perrott M. R. & Dunowska M. 2015. Primary possum macrophage cultures support the growth of a nidovirus associated with wobbly possum disease. J Virol Methods, 222, 66–71. https://doi.org/10.1016/j.jviromet.2015.05.014

Guo B.Q., Chen Z.S.& Liu W. X. 1996. [Isolation and Identification of PRRSV from aborted fetuses suspected of PRRS].Chinese J Prev Vet Med, 2, 1-5.

Harris L., Goyal S.M., Robison D., Christianson W.T., Morrison R.B., Gorcyca D. & Chladek D. 1992. Characterization of Swine Infertility and Respiratory Syndrome (SIRS) Virus (Isolate ATCC VR-2332). J Vet Diagn Invest, 4(2), 127-133. doi: 10.1177/104063879200400202.

He Y. L. 2012. [Lesions in Thymus of Piflets Infected with Highly Pathogenic PRRSV].Master Thesis. Chinese Academy of Agricultural Sciences.

Hopper S. A., White M. E. & Twiddy N. 1992. An outbreak of blue-eared pig disease (porcine reproductive and respiratory syndrome) in four pig herds in Great Britain. Vet Rec, 131(7), 140–144. https://doi.org/10.1136/vr.131.7.140

Hu S. P.,Zhang Z., Cai X.H., Tian Z.-J., Liu Y., An T. & Wu D.L. 2009. [Evaluation of protective efficacy of attenuated PRRS vaccine and inactivated mutant PRRSV strain]. Chinese J Prev Vet Med, 28, 392-396.

Kappes M.A. & Faaberg K.S. 2015. PRRSV structure, replication and recombination: Origin of phenotype and genotype diversity. Virology, 479, 475-486. doi: 10.1016/j.virol.2015.02.012

Lager K.M., Mengeling W L. & Brockmeier S L. 1997. Duration of homologous porcine reproductive and respiratory syndrome virus immunity in pregnant swine. Vet Microbiol, 58, 127-133.

Li Y. X. 2015. Brief Introduction to the Development of American Pig Industry. Journal China Animal Husbandry, 13, 56-59.

Liao C.Y. 2019. [Isolation and identification of PRRSV NADC30-Like CJS01 strain and establishment of its detection method]. Master's Dissertation, Sichuan Agricultural University.

Spilman M.S., Welbon C., Nelson E. & Dokland T. 2009. Cryo-electron tomography of porcine reproductive and respiratory syndrome virus: organization of the nucleocapsid. J Gen Virol, 90 (Pt 3),527-535.

Sun Z., Wang J., Bai X., Ji G., Yan H., Li Y., Wang Y., Tan F., Xiao Y., Li X. & Tian K. 2016. Pathogenicity comparison between highly pathogenic and NADC30-like porcine reproductive and respiratory syndrome virus. Arch Virol, 161(8), 2257-2261.

Tian K. 2017. NADC30-Like Porcine Reproductive and Respiratory Syndrome in China. Open Virol J,11,59-65.

Vu H.L X., Pattnaik A.K. & Osorio F.A. 2017. Strategies to broaden the cross-protective efficacy of vaccines against porcine reproductive and respiratory syndrome virus. Vet Microbiol, 206, 29-34.

Xiang M.Y., Liao C.Y., Jiang D.K., Zhang P., Wang Y., Luo Y., Yang Z.X. & Yao X.P. 2020. Establishment and Application of PRRSV NADC30-like SYBR Green I qPCR Detection Method. Biotechnology Bulletin, 36(5), 80-85. doi: 10.13560/j.cnki.biotech.bull.1985.2019-0885

Xu G.D. & Li Z.H. 2009. Progress in the prevention and treatment of porcine reproductive and respiratory syndrome in China. Journal China Animal Health, 1, 67-69.

Yin Z. & Liu J.H. 1997. Animal virology. 2nd edition. Scientific Publishing House, Beijing, China.

Yin, J., Zhang, H. and Qi, L. 2016. Research Advances on Effects of Porcine Reproductive and Respiratory Syndrome Virus on the Immune System of Pigs. Journal of Anhui Agricultural Sciences, 44, 81-82.

Zhang C.B. 2006. [The cloning and bioinformation analysis of ORF5 gene of PRRSV-SCQ strain & prokaryotic expression of dORF5 gene]. Master's Dissertation, Sichuan Agricultural University.

Zhang H. L., Tang D. Y. & Liu C. X. 2018. Adaptions of field PRRSVs in Marc-145 cells were determined by variations in the minor envelope proteins GP2a-GP3. Vet Microbiol, 222, 46-54.

Zhang H., Xia M., Wang W., Ju D., Cao L., Wu B., Wang X., Wu Y., Song N., Hu J., Tian C., Zhang S. & Wu H. 2018. An Attenuated Highly Pathogenic Chinese PRRS Viral Vaccine Confers Cross Protection to Pigs against Challenge with the Emerging PRRSV NADC30-Like Strain. Virol Sin, 33(2), 153-161.

Copyright (c) 2022 TENG TU, YIN WANG, CHANG YU LIAO, PENG FEI ZHANG, MING YUAN XIANG, ZE XIAO YANG, XUE PING YAO, YAN LUO