Biological and molecular genetic properties of the epizootic culture of Streptococcus equi isolated from pathological material of a foal
DOI:
https://doi.org/10.12834/VetIt.2744.18370.2Keywords:
Strangles, Strain, Antibiotic resistance, Therapy, Horse breedingAbstract
Strangles is one of the main constraining factors in the development of horse breeding. However, the veterinary practice still does not have effective drugs designed for the prevention and treatment of this disease. To date, the development of new effective methods and means for specific prevention and therapy of strangles is of scientific interest and meet the needs of the present day. In this regard, in the summer of 2020, the staff of the microbiology laboratory of the Research Institute of Biological Safety Problems brought samples (pus, exudate of submandibular lymph nodes, a smear of the oral and nasal cavities, a smear of the mucous membrane of the eye) from a foal sick with strangles from the village of Akterek, Jambyl district of Almaty region, and the strain was isolated. The aim of this article is to study molecular biological and genetic properties of Streptococcus equi isolated from pathological material of a foal. Streptococci were isolated by the inoculation of samples of pathological material on GRM-broth (nutrient medium for the cultivation of microorganisms), blood and GRM agar with the addition of 1% glucose and 10% sterile horse serum (pH 7.4-7.6). The results of bacteriological and molecular genetic studies confirmed that the isolate belongs to the species Streptococcus equi.
References
Agency for Strategic planning and reforms of the Republic of Kazakhstan. Bureau of National statistics. 2021. https://stat.gov.kz/.
Antonov B.I. (Ed.). 1987. Laboratory research in veterinary medicine: Viral, rickettsial and parasitic diseases. Agropromizdat, Moscow, 24.
Bizhanov A.B., Sansyzbayev A.R., Tokeyev Sh.O. 1994. The role of group C streptococci in equine
infectious diseases. Bull Selkhoznauk Kaz, 6, 121-3.
Dzhetigenov E.A., Bektashov A. & Aitbayev A. 2016. Cultural and morphological properties of isolates
of the horse washer pathogen. Bull Kyrgyz Natl Agrar Univ, 1, 190-4.
Dzhetygenov E.A. 2016. Epizootological features of horse strangles in Chui oblast. Bull Kyrgyz Natl Agrar Univ, 7, 48-53.
Espembetov B.A., Syrym N.S., Shestakov A.G. & Vasiliev D.A. 2019. Practical application of bacteriophages in the territory of the Republic of Kazakhstan. P.A. Stolypin Ulyanovsk State Agrarian University, Ulyanovsk, 624.
Guliy O.I., Karavayeva O.A., Larinova O.S., Larionov S.V., Lovtsova L.G., Uskov K.Yu. & Bunin V.D. 2018. Assessment of the effect of bacteriophages on microbial cells by electro-optical analysis. Antibiot Chemother, 63(1-2), 14-23.
Guliy O.I., Karavayeva O.A., Velikov V.A., Sokolov O.I., Pavliy S.A., Larionova O.S., Burov A.M. & Ignatov O.V. 2016. Study of the adsorption of bacteriophage FAb-Sp7 on the cell surface of Azospirillum brasilense Sp7. Virol Quest, 61(1), 45-8.
Isgren C.M., Williams N.J., Fletcher O.D., Timofte D., Newton R.J., Maddox T.W., Pinchbeck G.L. 2022. Antimicrobial resistance in clinical bacterial isolates from horses in the UK. Equine Vet J, 54(2), 390-414. https://doi.org/10.1111/evj.13437.
Jaramillo-Morales C., Gomez D.E., Renaud D. & Arroyo L.G. 2022. Streptococcus equi culture prevalence, associated risk factors and antimicrobial susceptibility in a horse population from colombia. J of Equine Vet Scien, 1, Article number 111.
Kaiypbai B.B. 2003. Treatment of a mixed course of strangles and pasteurellosis in horses: thesis of the Candidate of Veterinary Science. Kazakh Research Veterinary Institute, Almaty, 18.
Kutter E. & Sulakvelidze A. (Eds.). 2012. Bacteriophages: Biology and Applications. Nauchnyy mir, Moscow, 528.
Kutter E. 2001. Phage therapy: bacteriophages as antibiotics. Research Institute of Children’s Infections, Saint Petersburg, 945-56.
Lindahl S. 2013. Streptococcus equi subsp. equi and Streptococcus equi subsp. Zooepidemicus. SLU
Service/Repro, Uppsala, 75.
Newton J.R., Verheyen K., Talbot N.C., Lakhani K.H. & Chanter N. 2000. Control of strangles outbreaks by isolation of guttural pouch carriers identified using PCR and culture of Streptococcus
equi. Equine Vet J, 32(6), 515-26. https://doi.org/10.2746/042516400777584721.
Newton J.R., Wood J.L.N. & De Brauwere M.N. 1999. Detection and treatment of asymptomatic carriers of Streptococcus equi following strangles outbreaks in the UK. In: Equine Infectious Diseases VIII: Proceedings of the Eighth International Conference. R & W Publications, Newmarket, 82-87.
Pusterla N., Kass P.H., Mapes S., Whitehead B. & Manning J. 2011. Surveillance programme for important equine infectious respiratory pathogens in the USA. Vet Rec, 169(1), 12-3. http://dx.doi.org/10.1136/vr.d2157.
Sansyzbayev A.R. 1993. Washes horses in Kazakhstan, (distribution, properties of the pathogen, development of means of specific prevention and treatment): thesis of the Doctor of Veterinary Science. All-Russian Research Institute of Experimental Veterinary Medicine named after K.I. Skryabin and Ya.R. Kovalenko of the Russian Academy of Sciences, Moscow, 40.
Vasiliev D.A., Zolotukhin S.N. & Aleshkin A.V. (Eds.). 2013. Proceedings of the International Scientific and Practical Conference “Bacteriophages: Theoretical and Practical Aspects of Application in Medicine, Veterinary Medicine and Food Industry”. P.A. Stolypin Ulyanovsk State Agrarian University, Ulyanovsk, pp. 133-136.
Waller A.S. 2014. New perspectives for the diagnosis, control, treatment, and prevention of strangles in horses. Vet Clin North Am – Equine Pract, 30(3), 591-607. https://doi.org/10.1016/j.cveq.2014.08.007.
Wittebole X., de Roock S., Opal S.M. 2014. A historical overview of bacteriophage therapy as an alternative to antibiotics for the treatment of bacterial pathogens. Virulence, 5(1), 209-18.
Yespembetov B.A., Zinina N.N., Sarmykova M.K. & Syrym N.S. 2020. Results of the study of the epizootic culture of Streptococcus equi isolated from the pathological material of the foal. Biosaf Biotechnol, 1, 34-7.