Biological and molecular genetic properties of the epizootic culture of Streptococcus equi isolated from pathological material of a foal
DOI:
https://doi.org/10.12834/VetIt.2744.18370.2Keywords:
Strangles, Strain, Antibiotic resistance, Therapy, Horse breedingAbstract
Strangles is one of the main constraining factors in the development of horse breeding. However, the veterinary practice still does not have effective drugs designed for the prevention and treatment of this disease. To date, the development of new effective methods and means for specific prevention and therapy of strangles is of scientific interest and meet the needs of the present day. In this regard, in the summer of 2020, the staff of the microbiology laboratory of the Research Institute of Biological Safety Problems brought samples (pus, exudate of submandibular lymph nodes, a smear of the oral and nasal cavities, a smear of the mucous membrane of the eye) from a foal sick with strangles from the village of Akterek, Jambyl district of Almaty region, and the strain was isolated. The aim of this article is to study molecular biological and genetic properties of Streptococcus equi isolated from pathological material of a foal. Streptococci were isolated by the inoculation of samples of pathological material on GRM-broth (nutrient medium for the cultivation of microorganisms), blood and GRM agar with the addition of 1% glucose and 10% sterile horse serum (pH 7.4-7.6). The results of bacteriological and molecular genetic studies confirmed that the isolate belongs to the species Streptococcus equi.
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