Evaluation of Biosecurity Measures and Microbiological Quality of Table Eggs in Egyptian Layer Farms
DOI:
https://doi.org/10.12834/VetIt.3466.29418.2Keywords:
Biosecurity, Housing systems, Hygiene indicating bacteria, Layer farms, Table EggsAbstract
Table eggs are widely favored for their affordability, simplicity, and appeal across all age groups. They are a rich source of high-quality proteins, essential amino acids, minerals, and vitamins—nutrients vital for maintaining good health. The hygienic quality of table eggs is influenced by several factors, including the type of housing system and the level of biosecurity implemented on farms. This study evaluated the hygienic quality of table eggs produced in Egyptian layer farms, examining egg production across different housing systems and biosecurity levels. A total of 70 egg samples (both eggshell and egg content) were collected from seven layer farms representing diverse housing conditions and biosecurity standards. Samples were analyzed for total aerobic plate count (TAPC) and the presence of hygiene-indicating bacteria, including Staphylococcus, Pseudomonas, Escherichia coli, Salmonella, and Shigella. The results showed that farms with higher biosecurity scores (up to 97.5%), particularly those using closed battery systems, had significantly lower TAPC values and a reduced prevalence of pathogenic bacteria. In contrast, farms operating under open deep litter and backyard systems, with biosecurity scores of 30% and 22.5% respectively, exhibited notably higher microbial contamination. These findings underscore the crucial role of robust biosecurity practices and effective housing management in ensuring the hygienic quality of table eggs and protecting public health.References
Akbaş, E. (2014). National Microbiology Standards. Volume I: Infectious Diseases Laboratory Diagnostic Guideline; Ankara, Turkey.
Al-Shadeedi S. (2018). Study the Microbial Contamination of Table Egg Containers and Packages in Baghdad. 10th International Poultry Conference-Proceeding.
Arathy, S., Vanpee, G., Belot, G., Vanessa, M., Claude, D., &Ravindra, N.S. (2009). Bacterial contamination of commercial chicken eggs in Grenada. West Indies. West Indian Veterinary Journal, 2009; vol. 9, no. 2, pp. 4–7.
Attia, Y.A., Rahman, M.T., Hossain, M.J., Basiouni, S., Khafaga, A.F. Shehata,& Hafez, H.M. (2022). Poultry Production and Sustainability in Developing Countries under the COVID-19 Crisis: Lessons Learned. Animals, 12(5), 1-12.doi:10.3390/ani12050644.
Baron, F., Briandet, R., Lesne, J., Humbert, F., Ablain, W., &Gautier, M. (2004). Influence of a nonfavorable environment, egg white, on resistance to heat and disinfectant, adhesion, and virulence of Salmonella enteritidis. J Food Prot. 67(10):2269-73. doi: 10.4315/0362-028x-67.10.2269.
Carter, G.R., &Cole, R.J. (1990) Diagnostic procedures in veterinary bacteriology and mycology. 5 ed. San Diego, California: Academic Press Inc., pp: 307.
De Reu, K., Heyndrickx, M., Grijspreedt, K. (2007). Estimation of the vertical and horizontal bacterial infection of hen's table eggs. in Proc. 2007; 18th European Symposium on the Quality of Poultry Meat and 12th European Symposium on the Quality of Eggs and Egg Products, pp. 55-56, Prague, Czech Republic.
Dewulf, J., Postma, M., Van Immerseel, F., Vanbeselaere, B., &Luyckx, K. (2018). How to measure biosecurity and the hygiene status of farms. In Biosecurity in animal production and veterinary medicine: from principles to practice (Chapter 5 pp. 115–132). ACCO. https://doi.org/10.1079/9781789245684.0115.
El Ftouhy, F., Nacer, S., Nassik, S., &Hmyene A. (2021). The problem of campylobacter spp. in poultry farming. Moroccan Journal of Agronomic and Veterinary Sciences, vol. 9, no. 3, pp. 541–548, 2021.
European Communities (1993). Microbiological Criteria for Egg Products. Egg Products Regulation, http://www.legislation. gov.uk/uksi/1993/ 1520/made, 1993.
FAO, (2020). FAO Viet Nam urges improved application of biosecurity along poultry production chain. http://www.fao.org/vietnam/news/detail-events/en/c/1098535/.
Ferens, W.A., &Hovde, C.J. (2011). Escherichia coli O157:H7: animal reservoir and sources of human infection. Foodborne Pathog Dis. 8(4):465-87. doi: 10.1089/fpd.2010.0673.
Fikiin, K., Akterian, S., &Stankov, B. (2020). Do raw eggs need to be refrigerated along the food chain? Is the current EU regulation ensuring high-quality shell eggs for the European consumers? Trends in Food Science & Technology, 100: 359-362. Doi: 10.1016/j.tifs.2020.04.003.
Gast, R.K., &Holt, P.S. (2001). Multiplication in egg yolk and survival in egg albumen of Salmonella enterica serotype Enteritidis strains of phage types 4, 8, 13a, and 14b. J Food Prot. 2001 Jun; 64(6):865-8. doi: 10.4315/0362-028x-64.6.865.
Gast, R.K., Guard-Bouldin, J., &Holt, P.S. (2005). The relationship between the duration of fecal shedding and the production of contaminated eggs by laying hens infected with strains of Salmonella enteritidis and Salmonella Heidelberg. Avian Dis. 49(3):382-6. doi: 10.1637/7322-010705R.1.
Haeghebaert, S., Querrec, F.L., Gallay, A., Bouvet, Gomez, M., & Vaillant, V. (2002). Les toxi-infections alimentaires collectives en France, en 1999 et 2000. Bull. Epidemiol. Hebd 23:105–109.
Hafez, E. (2022). Effect of hygienic level on spreading of bacteria in poultry house. (Thesis) Ph. D. Animal, Poultry and environmental Hygiene. Fac. Vet. Med. Benha university.
Hamood, M.F., Jasim, H.N., & AL-Hassani, A.S.A. (2018). Evalution of contamination statuse in imported and local table eggs, Iraqi Journal of Agricultural Sciences, vol. 49, no. 3, pp. 388–393, 2018.
Hincke, M.T., Wellman-Labadie, O., McKee, M.D., Gautron, J., Nys, Y., &Mann, K. (2008). Biosynthesis and structural assembly of eggshell components, In: Mine, Y. (Ed.), Egg Bioscience and Biotechnology, John Wiley & Sons, New Jersey, pp. 97-128.
Ievy, S., Islam, M.S., Sobur, M.A., Talukder, M., Rahman, M.B., Khan, M.F.R.,& Rahman, M.T. (2020). Molecular Detection of Avian Pathogenic Escherichia coli (APEC) for the First Time in Layer Farms in Bangladesh and Their Antibiotic Resistance Patterns. Microorganisms.8(7):1021. doi: 10.3390/microorganisms8071021.
Indhu, B., Muthusami, S., &Thirunavukkarasu, N. (2014). Studies on Microflora and their Role on Eggshell Contamination and Infection. Int. J. Pharm. Chem. Bio. Sci., 4 (3): 518-521.
International Organization for Standardization (2004). Microbiology of food and animal feeding stuffs — Horizontal method for the detection of Shigella spp. ISO NO 21567:2004.
International Organization for Standardization (2017). Procedures for detection of Salmonella in animal faeces and in environmental samples from the primary production stage.ISO NO. 6579-1:2017.
Jambalang, A.R., Buys, E.M., & Both, F.S. (2017). Bacterial species from retailed poultry eggs in Tshwane, south Africa: implication for consumers. South African Journal of Science, vol. 113, pp. 1–7.
Jay, J.M., Golden, D.A., &Loessner, M.J. (2005). Modern Food Microbiology, Springer Verlag, Berlin, Germany, 7th edition.
Jones, F.T., Axtell, R., Rives, D.V., Scheideler, S.E., Tarver, J., Walker, R., &Wineland MJ. (1991). A survey of salmonella contamination in modern broiler production. J. Food Prot. 1991; 54: 502-507.
Kamboh, A.A., Shoaib, M., HAbro, S., AKhan, M., KMalhi, K., &Yu, S. (2018). Antimicrobial Resistance in Enterobacteriaceae Isolated from Liver of Commercial Broilers and Backyard Chickens. Journal of Applied Poultry Research. 27 (4): 627-634. Doi: 10.3382/japr/pfy045.
Kumar, M., Dahiya, S., &Ratwan, P. (2021). Backyard poultry farming in India: A tool for nutritional security and women empowerment. Biological Rhythm Research, 52(10), 1476-1491.
MacFaddin, J.F. (2000). Biochemical tests for identification medical bacteria. Warery Press Inc, Los Anglos, USA.
Mansour, A.F.A., Zayed, A.F., &Basha, O.A.A. (2015). Contamination of the shell and internal content of table eggs with some pathogens during different storage periods. Assiut Veterinary Medical Journal, 2015; vol. 61, pp. 8–15.
Mendes,F,R. (2010).Qualidadefísica, química e microbiológica de ovoslavadosarmazenados sob duastemperatuas e experimentalmentecontaminados com Pseudomonas aeruginosa. 2010. 72f. Dissertação (MestradoemCiência Animal) - Escola de Veterinária/Universidade Federal de Goiás, Goiânia.
Messens, W., Grijspeerdt, K., &Herman, L. (2006). Eggshell penetration of hen's eggs by Salmonella enterica serovar Enteritidis upon various storage conditions. Br Poult Sci. 47(5):554-60. doi: 10.1080/00071660600954601.
Methaq, G., Noor, A.,&Moutaz, A. (2020). study of bacterial contamination of defected egg shell and egg contents in Baghdad city. Plant Archives. 20) Supplement 1: 2306-2308.
Miyamoto, T., Horie, T., Baba, E., Sasai, K., Fukata, T., &Arakawa, A. (1998). Salmonella penetration through eggshell associated with freshness of laid eggs and refrigeration. J Food Prot. 61(3):350-3. doi: 10.4315/0362-028x-61.3.350.
Musgrove, M.T., Jones, D.R., Shaw, J.D., Sheppard, M., &Harrison, M.A. (2009). Enterobacteriaceae and related organisms isolated from nest run cart shelves in commercial shell egg processing facilities. Poult Sci. 88(10):2113-7. doi: 10.3382/ps.2009-00021.
Perez, R.H., &Ancuelo, A.E. (2022). Isolation and Characterization of Staphylococcus spp. and the Unintended Discovery of Non-Staphylococcal Strains Associated with Bovine Mastitis in Region IV-A, Philippines. Philippine Journal of Science, 151(5), 1793-1805.
Pinto, A.T., Mendonça, A.D., &Silva, E.N. (2009). Isolated or associated experimental contamination of albumen and egg yolk for Salmonella Enteritidis and Escherichia coli – influence of temperature and storage time. Arq. Bras. Med. Vet. Zootec. v.61, n.1, p.128-134. doi: 10.1590/S0102-09352009000100018.
Pondit, A., Haque, Z.F., Sabuj, A.A., Khan, M.S.R., &Saha, S. (2018). Characterization of Staphylococcus aureus isolated from chicken and quail eggshell. Journal of Advanced Veterinary and Animal Research, 5(4), 466–471. https://doi.org/ 10.5455/javar.2018.e300.
Racicot, M., &Vaillan court, J.P. (2009).Evaluation of biosecurity measures based on video surveillance in poultry farms in Quebec and main failures. Bulletin de l'AcadémieVétérinaire de France, 162(3), 265-272.
Radkowski, M. (2002). Effect of moisture and temperature on survival of Salmonella Enteritidis on shell eggs. Archiv Fur Geflugelkunde. 66, 119-123.
Rajmani, R.S., Verma, S.P. (2011). Microbial flora of eggs and egg contents from organized and unorganized poultry farms. Indian Journal of Veterinary Research. vol. 20, no. 1, pp. 73– 76.
Salihu, M.D., Garba,B.,&Isah, Y. (2015). Evaluation of microbial contents of table eggs at retail outlets in Sokoto metropolis. Sokoto Journal of Veterinary Sciences, 2015; vol. 13, no. 1, pp. 22–28.
Saliu, E., Vahjen, W., &Zentek, J. (2017). Types and prevalence of extended–spectrum beta–lactamase producing Enterobacteriaceae in poultry. Animal health research reviews, 18(1): 46-57. Doi: 10.1017/S1466252317000020.
Senbeta, E.K., Zeleke, N.A., &Molla, Y.G. (2015). Chemical composition and microbial loads of chicken table eggs from retail markets in urban settings of eastern Ethiopia. Journal of Advanced Veterinary and Animal Research, vol. 2, no. 4, pp. 404–409, 2015.
Sharma, D., Singh, N.K., Singh, H., Joachim, A., Rath, S.S.,& Blake, D.P. (2018). Discrimination, molecular characterisation and phylogenetic comparison of porcine Eimeria spp. in India. Vet Parasitol. 15;255:43-48. doi: 10.1016/j.vetpar.2018.03.020.
Soliman, E.S., &Abdallah, M.S. (2019). Assessment of biosecurity measures in broiler's farms in the Suez Canal area - Egypt using a seasonal prevalence of Salmonellosis. Vet World. 13(4):622-632. doi: 10.14202/vetworld.2020.622-632.
Sule, I.O., Olorunfemi, A.A., &Otori, A.O. (2019). Mycological and Bacteriological Assessment of Poultry Droppings from Poultry Pens within Ilorin, Kwara, Nigeria. Science World Journal. 14(4), 11-16.
Szafraniec, G.M., Szeleszczuk, P., &Dolka, B. (2022). Review on skeletal disorders caused by Staphylococcus spp. in poultry. Veterinary Quarterly, 42(1), 21-40.
Tanquilut, N.C., Espaldon, M.V.O., Eslava, D.F., Ancog, R.C., Medina, C.D.R., Paraso, M.G.V., Domingo, R.D., &Dewulf, J. (2020). Quantitative assessment of biosecurity in broiler farms using Biocheck.UGent in Central Luzon, Philippines. Poult Sci. 99(6):3047-3059. doi: 10.1016/j.psj.2020.02.004.
Tian, L., Hu, S., Jia, J., Tan, W., Yang, L., Zhang, Q., Liu, X.,& Duan X. (2021). Effects of short-term fermentation with lactic acid bacteria on the characterization, rheological and emulsifying properties of egg yolk. Food Chem. 30;341(Pt 1):128163. doi: 10.1016/j.foodchem.2020.128163.
Zakki, S.A., Qureshi, R., Hussain, A., Ghias, W., Sharif, M., &Ansari, F. (2017). Microbial quality evaluation and prevalence of bacteria and fungus in different varieties of chicken meat in Lahore. RADS Journal of Pharmacy and Pharmaceutical Sciences, 5(1), 30-37.
