TY - JOUR AU - R, Kalpana Devi AU - NV, Rajesh AU - S, Vasantha AU - N, Jeyathilakan PY - 2020/12/01 Y2 - 2024/03/29 TI - Screening of in vitro antitrematodal activities of compounds and secondary metabolites isolated from selected Pteridophytes JF - Veterinaria Italiana JA - VetIt VL - 56 IS - 4 SE - Paper DO - 10.12834/VetIt.1845.9825.4 UR - https://veterinariaitaliana.izs.it/index.php/VetIt/article/view/1845 SP - 271-287 AB - <p>Four ferns<em> Blechnum orientale Linn.</em> (BO), <em>Dicranopteris linearis</em> (Burm.f ) <em>Underw.</em> (DL), <em>Marattia fraxinea Sm.</em> (MF), and <em>Microlepia speluncae</em> (L.) Moore (MS) were extracted in varied combination of organic solvents followed by the preparation of eluates and isolation of secondary metabolites using chromatography on a glass column with silica gel as the fixed phase. The chemical components were identified using HPTLC and GC-MS analysis. The <em>in vitro</em> anti-trematodal activities of these eluates and compounds were evaluated against the sheep trematode worm <em>Gastrothylax crumenifer</em> (Plagiorrchiida: Gastrothylacidae) at increasing concentrations (1 to 5 mg/mL), to find the relative motility (RM) values for 0 to 60 min of incubation as reflection of paralysis and death of the worms. Hedon-Fleig salt solution was used as negative control and Oxyclozanide® 1% as standard control. <em>In vitro</em> incubation study showed DL and MS extracts had strong trematodicidal activity. BO extract (5 mg/mL) produced moderate trematodicidal activity and MF (5 mg/mL) showed the least trematodicidal activity. Phytochemicals analysis revealed that the ferns are a potential source of trematodicidal compounds such as phytol isomers fern-8-ene and fern-9(11)-ene (terpenoid derivative), quercetin 7,3 ́,4 ́-trimethoxy (flavonoid derivative), etc., which offer scope for a more elaborate study for exploitation of ferns for human welfare.</p> ER -