Abstract
The objective of this work was to characterize genetically some buffalo herds raised in Varzea (VA) and Terra-Firme (upland) (TF) ecosystems through polymorphism of the intron 2 of the leptin gene ( LEP-1620). Two hundred seventy-nine animals from four distinct populations were evaluated using the PCR-RFLP method for LEP-1620 polymorphism (SNP) of the leptin gene with restriction enzyme BsaAI. The animal samples were sorted into 4 groups, according to their breed and environmental origin: Mediterranean TF, Murrah TF, Mediterranean VA and Crossbreed VA. Two alleles (A and G) were detected and their frequencies were analyzed. Allele A frequency ranged from 0.395 (Mediterranean TF) to 0.850 (Murrah TF), with AA genotype ranged from 0.114 (Murrah TF) to 0.700 (Mediterranean TF). The observed and expected heterozygosities ranged from 0.268 (Mediterranean TF) to 0.562 (Murrah TF), and 0.255 (Mediterranean TF) to 0.478 (Murrah TF), respectively. The Hardy-Weinberg probabilities were greater than 0.05. The Crossbred herd in Varzea was the only population with significant inbreeding and the Shannon index ranged between 0.423 (Mediterranean TF) and 0.671 (Murrah TF).
References
Albuquerque M.D.S.M., Egito A.D., Marques J.R.F., Ciampi A.Y., Mariante A.D.S., Castro S.T.R., Costa M.R., Paiva S.R., Silva A.M. & Contel E.P.B. 2006. Variabilidade genética em búfalos estimada por marcadores RAPD. Pesq Agropec Bras, 41, 623-628.
Attia M., Abou-Bakr S. & Nigm A. A. 2014. Genetic differentiation and relationship among egyptian nile delta located buffalo using microsatellite markers. Egyptian J Anim Prod, 51, 71-77.
Azari M.A., Hasani S., Heidari M. & Yousefi S. 2012. Genetic polymorphism of leptin gene using PCR-RFLP method in three different populations. Slovak J Anim Sci, 45, 39-42.
Barbosa E.M., Souza B.B., Guimarães R.C., Azevedo J.S.N., Gonçalves E.C., Ribeiro H.F.L., Rolim Filho S.T. & Silva Filho E. 2016. Polymorphism in the melatonin receptor gene in buffalo populations of the Brazilian Amazon. Genet Mol Res, 15, 1-6.
Barros E.A., Ribeiro M.N., Almeida M.J.O. & Araújo A. M. 2011. Estrutura populacional e variabilidade genética da raça caprina Marota. Arch Zootec, 60, 543-552.
Catunda A.G.V., Lima F.R.G., Lima I.C.S., Machado A.A.C., Gadelha C.R.F., Pereira E.S., Martins G.A. & Campos A.C.N. 2014. O papel da leptina na reprodução dos ruminantes. Rev Bras Reprod Anim, 38,3-9.
Campanile G., Neglia G. & Michael J.D. 2016. Embryonic and fetal mortality in river buffalo (Bubalus bubalis). Theriogenology, 86, 207-213.
Giblin L., Butler S.T., Kearney B.M., Waters S.M., Callanan M.J. & Berry D.P. 2010. Association of bovine leptin polymorphisms with energy output and energy storage traits in progeny tested Holstein-Friesian dairy cattle sires. BMC Genet, 11,73.
Instituto Brasileiro de Geografia e Estatística (IBGE). 2014. (https://sidra.ibge.gov.br/home/pms/brasil accessed on 14 april 2016)
Kowalski L.H., Freitas J.H., Fernandes S.R., Junior P.R., Fernandes J.I.M. & Da Silva M.G.B. 2014. Leptina e grelina na produção de ruminantes. Rev Cien Agrarias, 37, 375-383.
Lara M.A.C., Pinatti E., Faria M.H., Resende F.D., Piveta A.J., Gutmanis G. & Cavalcante-Neto A. 2011. Polimorfismo do gene leptina (SNP305) em bovinos e sua implicação na maciez de carne. Actas Iberoamericanas de Conservación Animal, 1, 195-198
Lien S., Sundvold H., Klungland H. & Vage D.I. 1997. Two novel polymorphisms in the bovine obesity gene (OBS). Anim Genet, 28, 238–246.
Ludwig J. A., Quartet L. & Reynolds J.F. 1988. Statistical ecology: A Primer on Methods and Computing. Wiley-Interscience Pub, New York.
Marcondes R. & Righetti C. 2011. Melhoramento de búfalos no Brasil: avanços, entraves e perspectivas. Rev Bras Zootec, 40, 325-333.
Marques J.R.F., Martínez A.M., Costa M.R., Albuquerque M.S.M., Quiroz J., Vega-Pla J.L. & Delgado J.V. 2011. Diversidade genética de búfalos brasileiros (Bubalus bubalis) utilizando microssatélites de DNA. Arch Zootec, 60, 1213-1221.
Mishra B.P., Kataria R.S., Kathiravan P., Bulandi S.S., Singh K.P. & Sadana, D.K. 2009. Evaluation of genetic variability and mutation drift equilibrium of Banni buffalo using multi locus microsatellite markers. Trop Anim Health Prod, 41, 1203-1211.
Nei M. 1972. Genetic distance between populations. American Naturalist, 106, 283-292.
Nei M. 1978. Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics, 89, 583-590.
Pérez-Pérez A., Sánchez-Jiménez F., Maymó J., Dueñas J.L., Varone C. & Sánchez-Margalet V. 2015. Role of leptin in female reproduction. Clin Chem Lab Med, 53, 15-28.
Ramalho R.D.O.S., Rodrigues V.C., Do Couto D.M., Pitombo R.S., De Souza D.D.N. & De Araújo A.H.B. 2013. Medidas corporais e características de carcaça de bubalinos Mediterrâneo castrados e inteiros. Boletim Indústria animal, 70, 20-27.
Raymond M. & Rousset F. 1995. GENEPOP Version 1.2: population genetics software for exact tests and ecumenicism. J Hered, 86, 248-249.
Rodrigues A.E., Marques J.R.F., Araújo, C.V., Camargo Júnior, R.N.C., Dias, L.N.S. 2010 Estimação de parâmetros genéticos para características produtivas em búfalos na Amazônia Oriental. Arq Bras Med Vet Zootec, 62, 712-717.
Rodrigues F.B., Carneiro P.L.S., Ramos A.A., Ambrosini D.P. & Malhado C.H.M. 2015. Interação genótipo x ambiente para peso aos 365 dias em bubalinos da raça Mediterrâneo. Pesq Agropec Bras, 50, 615-621.
Sambrook, J., Fritsch, E.F. & Maniatis, T. 1989. Molecular Cloning: a laboratory manual. Cold Spring Harbor Laboratory, New York.
Silva C.S., Silva Filho E., Matos A.S., Schierholt A.S., Costa M.R., Marques L.C., Costa J.S., Sales R.L., Figueiró M.R. & Marques J.R.F. 2016. Polymorphisms in the DGAT1 gene in buffaloes (Bubalus bubalis) in the Amazon. Genet Mol Res, 15, 1-7.
Souza F.R.P., Mercadante M.E.Z., Fonseca L.F.S., Ferreira L.M.S., Regatieri I.C.; Ayres D. R., Tonhati H., Silva S.L., Razook, A.G. & Albuquerque L.G. 2010. Assessment of DGAT1 and LEP gene polymorphisms in three Nelore (Bos indicus) lines selected for growth and their relationship with growth and carcass traits. J Anim Sci, 88, 435-441.
Uramoto K., Walder J. M.M. & Zucchi R.A. 2005. Análise quantitativa e distribuição de populações de espécies de Anastrepha (Diptera: Tephritidae) no campus Luiz de Queiroz, Piracicaba, SP. Neotropical Entomol, 34, 33-39.
Yeh F.C., Yang R.C., Boyle T. B.J., Ye Z.H. & Mao J.X. 1997. POPGENE, The User-Friendly Shareware For Population Genetic Analysis. Molecular Biology and Biotechnology Centre, University of Alberta, Canada.
Wright S. 1965. The interpretation of population structure by F‐statistics with special regard to systems of mating. Evolution, 19, 395-420.
Zetouni L., Camargo G.M.F., Fonseca P.D.S., Monsalves F.M., Lugo A.H., Aspilcueta-Borquis R.R.& Cervini M. 2013. Effects of a single nucleotide polymorphism in the leptin gene on the productive traits of dairy buffaloes (Bubalus bubalis). Mol Biol Rep, 40, 5159-5163.