Abstract
The Authors investigated polymorphism of the bovine BoLA‑DRB3 gene in connection with resistance or susceptibility to brucellosis of two Kazakh meat breeds, Auliekol and Kazakh Whiteheaded breeds, using PCR‑RFLP. In Auliekol cattle (n = 158), 22 alleles were detected in the brucellosis group, and 24 alleles were shown in the healthy group. BoLA-DRB3 alleles *3, *4, *19, *21 were more common in healthy animals, while Brucella‑positive cattle were more frequently carriers of alleles *7, *10, *18. In Kazakh Whiteheaded cattle (n = 146), 21 alleles were detected in infected and 23 alleles in healthy cattle. Alleles *3, *8, *21 significantly predominate in healthy cattle, while alleles *7, *11, *16 are typical for animals with brucellosis. This study identified BoLA-DRB3 alleles associated with genetic resistance (*3 and *21) and susceptibility (*7) to brucellosis; remarkably, resistance alleles are shared by two important meat breeds of Kazakhstan.
References
Ballingall K.T., Luyai A., Rowlands G.J., Sales J., Musoke A.J., Morzaria S.P. & McKeever D.J. 2004. Bovine Leukocyte Antigen Major Histocompatibility Complex Class II DRB3*2703 and DRB3*1501 alleles are associated with variation in levels of protection against Theileria parva challenge following Immunization with the sporozoite p67 antigen. Infect Immun, 72(5), 2738-2741.
Corbel MJ. 1997. Brucellosis: an overview. Emerg Infect Dis, 3, 213–221.
Gordienko L. N. 2014. The intensity of the spread of brucellosis in cattle meat breed under range content. Vet Sci, 11, 17-20.
Gordienko L. N., Kulikova E. V., Gaidutskaya G. M., Elantseva N. B., Volkova T. I. & Guskova T. V. 2017. Assessment of the immune status of imported cattle treated for brucellosis. Veterinary, 2, 19-22.
Gulyukin M. I., Albertyan M. P., Iskandarov M. I., Fedorov A. I &, Kolomytsev S. A. 2013. Brucellosis of farm animals in the Russian Federation. Veterinary, 6, 23-28.
Gulyukin M. I., Albertyan M. P., Iskandarov M. I., Fedorov A. I. & Iskandarova S. S. 2016. Effectiveness of measures taken against bovine brucellosis in the Russian Federation.Veterinary,12, 24-28.
Dospekhov B.A. 1985. Field experiment methodology (with the basics of statistical processing of research results). M Agropromizdat, 352.
Ivanov A.V., Salmakov K. M., Fomin A. M. 2013. State and prospects of specific prevention and elimination of brucellosis of cattle. Veterinary, 7, 10-13.
Kerimov C.H. & Iskandarov M.I. 1999. Prevention and improvement of camels and cattle from brucellosis. Development of animal husbandry scientific support, Ashgabat
Latypova Z., Sarbakanova Sh., Sultanov A., Nam I., Zayakin V., Achmedov R., Smaznova I., Egiazaryan A., Sheiko I. & Budevich A. 2017. DNA-polymorphism of the BoLA-DRB3 gene in crossbreeding of Holstein and Black Pied cattle from the Bryansk region of Russia, Belarus and Kazakhstan. Current Science, 5, 2173 – 2186.
Lewin H. A. 1989. Disease resistance and immune response genes in cattle: strategies for their detection and evidence of their existence. J Dairy Sci, 72(5), 1334-1348.
Lewin H.A., Russel G.C. & Glass E.J. 1999. Comparative organization and function of the major histicompatibility complex of domesticated cattle. Immunol Rev, 167, 145-158.
Maillard J.C., Martinez D. & Bensaid A. 1996. An amino acid sequence coded by the exon 2 of the BoLA DRB3 gene associated with a BoLA class I specificity constitutes a likely genetic marker of resistance to dermatophilosis in Brahman Zebu cattle of Martinique (FWI). Ann New York Acad Sci, 791, 185–197.
Jean-Charles M., Berthier D., Chantal I., Thevenon S., Sidibй I., Stachurski F., Belemsaga, Hanta Razafindraпbi D. & Elsen JM. 2003. Selection assisted by a BoLA-DR/DQ haplotype against susceptibility to bovine dermatophilosis. Genet Sel Evol, 35(1), S193–S200.
Nam I.Y., Zayakin V.V., Smaznova I.A., Egiazaryan A.V., Sulimova G.E., Sheiko I.P. & Budevich A.I. 2014. High Genetic Susceptibility to Leukemia in Breeding Black Pied and Holstein Cattle. Middle-East Journal of Scientific Research, 20(10), 1297-1301.
Nam I.Y., Zayakin V.V., Smaznova I.A., Kozlov A.L., Achmedov R.B. & Kobozeva M.S. 2015. The genetic polymorphism of BoLA-DRB3 gene and the resistance to virus leukemia in different herds of cattle at Bryansk region. Res J Pharmac Biol Chem Sci, 6(1), 1903-1907.
Nam I.a., Zayakin V.V. & Drozdov E.V. 2014. Allelic polymorphism of the somatotropin gene among Holstein-friesian breed in the livesock farming in the Bryansk region. World Appl Sci J, 30(7), 802- 805.
Nassiry M.R., Eftekhar Shahroodi F., Mosafer J., Mohammadi A., Manshad E., Ghazanfari S., Mohammad Abadi M.R. & Sulimova G.E. 2005. Analysis and frequency of bovine lymphocyte antigen (BoLA-DRB3) alleles in Iranian holstein cattle. Genetics, 41(6), 817-822.
Satsuk V. F. 2009. Using the Bola-DRB3 marker in the selection and breeding work with cattle. Cand Dissertation in Biol Sci, Stavropol, 118 p.
Smaznova I. A. 2015. Characteristics of allelic polymorphism of the BoLA-DRB3 gene, which affects resistance to leukemia, and genes of milk productivity in producing bulls of the Bryansk region. PhD Thesis, Saint Petersburg, 126 p.
Sulimova G.E., Sokolova S.S., Semikozova O.P., Nguet L.M. & Berberov E.M. 1992. Analysis of DNA polymorphism of cluster genes in cattle: casein genes and major histocompatibility complex (MHC) genes. Genetika, 26(5), 18-26.
Sulimova G.E., Udina I.G., Shaïkhaev G.O. & Zakharov I.A. 1995. DNA polymorphism of the BoLA-DRB3 gene in cattle in connection with resistance and susceptibility to leukemia. Genetika, 31(9), 1294-1299.
Suprovych T., Suprovych N., Karchevska T., Chornyy I. & Chepurna V. 2016. Allelic polymorphism gene BOLA-DRB3.2 in connection with the types of excretory ducts udder and mastitis cows Ukrainian black-pied dairy breed. Scientific Messenger LNUVMBT named after S.Z. Gzhytskyj, 3(71), 117–123. https://nvlvet.com.ua/index.php/journal/article/view/943
Sultanov A. A. 2012. Veterinary issues of development of meat cattle breeding in Kazakhstan. Agrarian sector, 1, 11.
Usmanov R.R. & Proshina E.T. 2013. Features of statistical processing of field experience: Study guide. M .: FSBEI of AE FCEBC, 96.
Van Eijk M.J., Stewart-Haynes J.A., Lewin H.A. 1992. Extensive polymorphism of the BoLA-DRB3 gene distinguished by PCR-RFLP. Anim Genet, 23(6), 483-496.
Xu A., Van Eijk M.J., Park C., Lewin H.A. 1993. Polymorphism in BoLA-DRB3 exon 2 correlates with resistance to persistent lymphocytosis caused by bovine leukemia virus. J Immunol, 151(12), 6977-6985.