Molecular identification of Cryptosporidium andersoni in healthy and in cattle with diarrhea of Mashhad, Northeast of Iran
VetIt.1771.9343.3.pdf

Supplementary Files

Table I. Prevalence of Cryptosporidium spp. infection in cattle by age in Mashhad, Northeast of Iran
Table II. Prevalence of Cryptosporidium spp. infection in healthy and diarrheic cattle in Mashhad, Northeast of Iran

Keywords

Cryptosporidium andersoni
Cattle
PCR‑RFLP
Mashhad
Iran

How to Cite

Sadrebazzaz, A. (2020). Molecular identification of Cryptosporidium andersoni in healthy and in cattle with diarrhea of Mashhad, Northeast of Iran. Veterinaria Italiana, 56(1), 43-46. https://doi.org/10.12834/VetIt.1771.9343.3

Abstract

Cryptosporidium is an intracellular and extracytoplasmic protozoan that belongs to the phylum Apicomplexa. In this observational study, fecal samples were randomly collected from 800 dairy cattle, in 10 industrial dairy farms in Mashhad, Iran (from 2011 to 2015 years). The presence of Cryptosporidium oocysts was determined by modified cold Ziehl‑Neelsen’s staining. Results of microscopy observation showed that 23 samples (2.87%) were positive for Cryptosporidium oocyst. Twenty two samples were confirmed by PCR. The identification ofCryptosporidium andersoni was determined by restriction digestions of PCR products, using SspI, VspI, and DdeI enzymes. Differences between healthy and diarrheic groups as well as between age groups were not observed.
https://doi.org/10.12834/VetIt.1771.9343.3
VetIt.1771.9343.3.pdf

References

Blears M.J., Pokorny N.J., Carreno R.A., Chen S., De Grandis S.A., Lee H. & Trevors J.T. 2000. DNA fingerprinting of Cryptosporidium parvum isolates using amplified fragment length polymorphism (AFLP). J Parasitol, 86, 838‑841.

Burnet J.B., Ogorzaly L., Tissier A., Penny C. & Cauchie H.M. 2013. Novel quantitative TaqMan real‑time PCR assays for detection of Cryptosporidium at the genus level and genotyping of major human and cattle‑infecting species. Journal of Applied Microbiology, 114, 1211‑1222.

Dillingham R.A., Lima A.A. & Guerrant R.L. 2002. Cryptosporidiosis: epidemiology and impact. Microbes Infect, 4, 1059‑1066.

Fayer R. & Xiao L. 2008. Cryptosporidium and cryptosporidiosis. Boca Raton: Taylor & Francis Group.

Fiuza V.R., Almeida A.J., Frazao‑Teixeira E., Santin M., Fayer R. & Oliveira F.C. 2011. Occurrence of Cryptosporidium andersoni in Brazilian cattle. J Parasitol, 97, 952‑953.

Fotouhi Ardakani R.F.H.M., Solayman Banai S., Kamyabi H., Atapour M. & Sharifi I. 2008. Epidemiology of Cryptosporidium infection of cattle in Kerman/Iran and molecular genotyping of some isolates. Journal of Kerman University of Medical Sciences, 15, 313‑320.

Gatei W., Greensill J., Ashford R.W., Cuevas L.E., Parry C.M., Cunliffe N.A., Beeching N.J. & Hart C.A. 2003. Molecular analysis of the 18S rRNA gene of Cryptosporidium parasites from patients with or without human immunodeficiency virus infections living in Kenya, Malawi, Brazil, the United Kingdom, and Vietnam. J Clin Microbiol, 41, 1458‑1462.

Koyama Y., Satoh M., Maekawa K., Hikosaka K. & Nakai Y. 2005. Isolation of Cryptosporidium andersoni Kawatabi type in a slaughterhouse in the northern island of Japan. Vet Parasitol, 130, 323‑326.

Kvac M. & Vitovec J. 2003. Prevalence and pathogenicity of Cryptosporidium andersoni in one herd of beef cattle. J Vet Med B Infect Dis Vet Public Health, 50, 451‑457.

Mendonca C., Almeida A., Castro A., de Lurdes Delgado M., Soares S., da Costa J.M. & Canada N. 2007. Molecular characterization of Cryptosporidium and Giardia isolates from cattle from Portugal. Vet Parasitol, 147, 47‑50.

Morgan U.M., Constantine C.C., O'Donoghue P., Meloni B.P., O'Brien P.A. & Thompson R.C. 1995. Molecular characterization of Cryptosporidium isolates from humans and other animals using random amplified polymorphic DNA analysis. Am J Trop Med Hyg, 52, 559‑564.

Nouri M., Razmyar J. & Keyhani P. 1995. A Cryptosporidium muris like parasite in large ruminants in various parts of Iran. Journal of Faculty of Veterinary Medicine University of Tehran, 50, 1‑5.

Nouri M. & Khalaji M.R. 2003. A study of possible existence of Cryptosporidium muris‑like (andersoni) and it's abdominal pathologic changes in dairy cattle around Isfahan and role of mice and water in transmission. Journal of Veterinary Research, 58, 37‑40.

Ondrackova Z., Kvac M., Sak B., Kvetonova D. & Rost M. 2009. Prevalence and molecular characterization of Cryptosporidium spp. in dairy cattle in South Bohemia, the Czech Republic. Vet Parasitol, 165, 141‑144.

Paul S., Chandra D., Tewari A.K., Banerjee P.S., Ray D.D., Raina O.K. & Rao J.R. 2009. Prevalence of Cryptosporidium andersoni: a molecular epidemiological survey among cattle in India. Vet Parasitol, 161, 31‑35.

Putignani L. & Menichella D. 2010. Global distribution, public health and clinical impact of the protozoan pathogen cryptosporidium. Interdiscip Perspect Infect Dis, 2010, pii: 753512.

Robinson G., Thomas A.L., Daniel R.G., Hadfield S.J., Elwin K. & Chalmers R.M. 2006. Sample prevalence and molecular characterisation of Cryptosporidium andersoni within a dairy herd in the United Kingdom. Vet Parasitol, 142, 163‑167.

Silverlas C., Naslund K., Bjorkman C. & Mattsson J.G. 2010. Molecular characterisation of Cryptosporidium isolates from Swedish dairy cattle in relation to age, diarrhoea and region. Vet Parasitol, 169, 289‑295.

Tahvildar‑Biderouni F. & Salehi N. 2014. Detection of Cryptosporidium infection by modified ziehl‑neelsen and PCR methods in children with diarrheal samples in pediatric hospitals in Tehran. Gastroenterol Hepatol Bed Bench, 7, 125‑130.

Tyzzer E.E. 1907. A sporozoan found in the peptic glands of the common mouse. Proc Soc Exp Biol Med, 5, 12‑13.

Wang R., Wang H., Sun Y., Zhang L., Jian F., Qi M., Ning C. & Xiao L. 2011. Characteristics of Cryptosporidium transmission in preweaned dairy cattle in Henan, China. J Clin Microbiol, 49, 1077‑1082.

Xiao L., Escalante L., Yang C., Sulaiman I., Escalante A.A., Montali R.J., Fayer R. & Lal A.A. 1999. Phylogenetic analysis of Cryptosporidium parasites based on the small‑subunit rRNA gene locus. Appl Environ Microbiol, 65, 1578‑1583.

Xiao L., Alderisio K., Limor J., Royer M. & Lal A.A. 2000. Identification of species and sources of Cryptosporidium oocysts in storm waters with a small‑subunit rRNA‑based diagnostic and genotyping tool. Appl Environ Microbiol, 66, 5492‑5498.

Xiao L., Bern C., Limor J., Sulaiman I., Roberts J., Checkley W., Cabrera L., Gilman R.H. & Lal A.A. 2001. Identification of 5 types of Cryptosporidium parasites in children in Lima, Peru. J Infect Dis, 183, 492‑497.