Molecular identification of Cryptosporidium andersoni in healthy and in cattle with diarrhea of Mashhad, Northeast of Iran

Supplementary Files

Table I. Prevalence of Cryptosporidium spp. infection in cattle by age in Mashhad, Northeast of Iran
Table II. Prevalence of Cryptosporidium spp. infection in healthy and diarrheic cattle in Mashhad, Northeast of Iran


Cryptosporidium andersoni

How to Cite

Sadrebazzaz, A. (2020). Molecular identification of Cryptosporidium andersoni in healthy and in cattle with diarrhea of Mashhad, Northeast of Iran. Veterinaria Italiana, 56(1), 43-46.


Cryptosporidium is an intracellular and extracytoplasmic protozoan that belongs to the phylum Apicomplexa. In this observational study, fecal samples were randomly collected from 800 dairy cattle, in 10 industrial dairy farms in Mashhad, Iran (from 2011 to 2015 years). The presence of Cryptosporidium oocysts was determined by modified cold Ziehl‑Neelsen’s staining. Results of microscopy observation showed that 23 samples (2.87%) were positive for Cryptosporidium oocyst. Twenty two samples were confirmed by PCR. The identification ofCryptosporidium andersoni was determined by restriction digestions of PCR products, using SspI, VspI, and DdeI enzymes. Differences between healthy and diarrheic groups as well as between age groups were not observed.


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