Peste des petits ruminants in Nigeria: identification and variations of lineage II and lineage IV in sheep and goats

Authors

  • Ijeoma Chekwube Chukwudi Department of Veterinary Medicine, University of Nigeria Nsukka, Enugu State, Nigeria
  • Francesco Mira Istituto Zooprofilattico Sperimentale della Sicilia "A.Mirri"
  • Kenneth Ikejiofor Ogbu Department of Animal Health, Federal College of Animal Health and Production Technology, National Veterinary Research Institute Vom, Plateau State, Nigeria
  • Refiloe Petunia Malesa Transboundary Animal Disease Laboratory, Agricultural Research Council-Onderstepoort Veterinary Institute, Onderstepoort, South Africa
  • Giuseppa Purpari Istituto Zooprofilattico Sperimentale della Sicilia “A. Mirri”, Palermo, Italy
  • Pam Dachung Luka Applied Biotechnology Division, National Veterinary Research Institute Vom, Plateau State, Nigeria
  • Emmanuel Ikenna Ugochukwu Department of Veterinary Medicine, University of Nigeria Nsukka, Enugu State, Nigeria
  • Livio Edward Heath Transboundary Animal Disease Laboratory, Agricultural Research Council-Onderstepoort Veterinary Institute, Onderstepoort, South Africa
  • Annalisa Guercio Istituto Zooprofilattico Sperimentale della Sicilia “A. Mirri”, Palermo, Italy
  • Kennedy Foinkfu Chah Department of Veterinary Pathology and Microbiology, University of Nigeria Nsukka, Enugu State, Nigeria

DOI:

https://doi.org/10.12834/VetIt.2390.14842.1

Keywords:

Genetic characterization, Goat, Nigeria, Peste‑des‑petit‑ ruminants virus, Lineage II, Lineage IV, Sheep, Small ruminant morbillivirus

Abstract

Peste des petits ruminants virus (PPRV) is the aetiologic agent of Peste des petits ruminants (PPR), an important viral disease of sheep and goats. PPR is endemic in Nigeria and leads to social and economic losses. The objective of this study was to determine the prevalence of PPR infection and genetically characterize PPRV strains obtained from sheep and goats in three States of Southeast Nigeria. A total of 285 nasal swab samples collected in 2017‑2018 were processed for PPRV genome detection by RT‑PCR. Sixty‑five (22.81%) of the samples were positive for PPRV. Sequence and phylogenetic analyses revealed that the PPRV belonged to lineages II (11/38, 28.9%) and IV (27/38, 71.1%). The N gene fragment sequence showed a
99.77%‑100% and 99.98%‑100% identity among the strains of lineages II and IV, respectively. Fourteen amino acid substitutions, previously unreported in PPRV strains from Nigeria, were recorded. This study confirms the circulation of PPRV lineages II and IV in Southeast Nigeria, the dominance of strains belonging to lineage IV in recent years, and their close genetic relationship with those previously reported in other parts of Nigeria and neighboring countries.

References

Abu Elzein E.M., Hassanien M.M., Al-Afaleq A.I., Abd Elhadi M.A. & Housawi F.M. 1990. Isolation of peste des petits ruminants from goats in Saudi Arabia. Vet Rec, 127, 309–310.

Al-Majali A.M., Hussain N.O., Amarin N.M. & Majok A.A. 2008. Seroprevalence of, and risk factors for, peste des petits ruminants in sheep and goats in Northern Jordan. Prev Vet Med, 85, 1–8.

Amarasinghe G.K., Ayllón M.A., Bào Y., Basler C.F., Bavari S. & Blasdell K.R. 2019. Taxonomy of the order Mononegavirales: update 2019. Arch Virol, 164, 1967–1980.

Anderson J., McKay J.A. & Butcher R.N. 1991. The use of monoclonal antibodies in competitive ELISA for the detection of antibodies against Rinderpest and Peste des petits ruminants virus. In Proc. Final research co-ordination meeting on the sero-monitoring of rinderpest throughout Africa, Bingerville (Cote d'Ivoire), 19-23 Nov 1990. International Atomic Energy Agency, Vienna, Austria.

Baazizi R., Mahapatra M., Clarke B.D., Ait-Oudhia K., Khelef D. & Parida S. 2017. Peste des petits ruminants (PPR): A neglected tropical disease in Maghreb region of North Africa and its threat to Europe. PLoS ONE, 12, e0175461.

Bailey D., Banyard A., Dash P., Ozkul A. & Barrett T. 2005. Full genome sequence of peste des petits ruminants virus, a member of the Morbillivirus genus. Virus Res, 110, 119–124.

Banyard A.C., Parida S., Batten C., Oura C., Kwiatek O. & Libeau G. 2010. Global distribution of peste des petits ruminants virus and prospects for improved diagnosis and control. J Gen Virol, 91, 2885–2897.

Chukwudi I.C., Ogbu K.I., Nwabueze A.L., Olaolu O.S., Ugochukwu E.I. & Chah K.F. 2020. Update on Peste des petits ruminants status in South East Nigeria: serological and farmers’ awareness investigation, and potential risk factors. Trop Anim Health Prod, 52(6), 3285-3291

Cosseddu G.M., Pinoni C., Polci A., Sebhatu T., Lelli R. & Monaco F. 2013. Characterization of peste des petits ruminants virus, Eritrea, 2002-2011. Emerging Infect Dis, 19, 160–161.

Couacy-Hymann E., Bodjo C., Danho T., Libeau G. & Diallo A. 2005. Surveillance of wildlife as a tool for monitoring rinderpest and peste des petits ruminants in West Africa. Rev Sci Tech, 24(3), 869-77 Rev - Off Int Epizoot, 24, 869–877.

Couacy-Hymann E., Roger F., Hurard C., Guillou J.P., Libeau G. & Diallo A. 2002. Rapid and sensitive detection of peste des petits ruminants virus by a polymerase chain reaction assay. J Virol Methods, 100, 17–25.

Devi M., Das S., Sharma K. & Dutta R. 2016. Seroprevelance and molecular detection of peste des petits ruminants in goats of Assam. Virusdisease, 27, 91–97.

Dhar P., Sreenivasa B.P., Barrett T., Corteyn M., Singh R.P. & Bandyopadhyay S.K. 2002. Recent epidemiology of peste des petits ruminants virus (PPRV). Vet Microbiol, 88, 153–159.

Donduashvili M., Goginashvili K., Toklikishvili N., Tigilauri T., Gelashvili L., Avaliani L., Khartskhia N., Loitsch A., Bataille A., Libeau G., Diallo A. & Dundon WG. 2018. Identification of Peste des Petits Ruminants Virus, Georgia, 2016. Emerging Infect Dis, 24, 1576–1578.

Dundon W.G., Diallo A. & Cattoli G. 2020. Peste des petits ruminants in Africa: a review of currently available molecular epidemiological data, 2020. Arch Virol, 165, 2147–2163.

Ezeokoli C.D., Umoh J.U., Chineme C.N., Isitor G.N. & Gyang E.O. 1986. Clinical and epidemiological features of peste des petits ruminants in Sokoto Red goats. Rev Elev Med Vet Pays Trop, 39, 269–273.

Ezeibe M.C.O., Okoroafor O.N., Ngene A.A., Eze J.I., Eze I.C. & Ugonabo J. A. 2008. Persistent detection of peste de petits ruminants antigen in the faeces of recovered goats. Trop Anim Health Prod, 40, 517–519.

Forsyth M.A. & Barrett T. 1995. Evaluation of polymerase chain reaction for the detection and characterisation of rinderpest and peste des petits ruminants viruses for epidemiological studies. Virus Res, 39, 151–163.

Gargadennec L. & Lalanne A. 1942. La Peste des petits ruminants. Bull Serv Zoo A O, 5, 16–21

Gibbs E.P., Taylor W.P., Lawman M.J. & Bryant J. 1979. Classification of peste des petits ruminants virus as the fourth member of the genus Morbillivirus. Intervirology, 11, 268–274.

Kumar K. Senthil, Babu A., Sundarapandian G., Roy P., Thangavelu A., Kumar K. S., Arumugam R., Chandran N.D., Muniraju M., Mahapatra M., Banyard A.C., Manohar B.M. & Parida S. 2014. Molecular characterisation of lineage IV peste des petits ruminants virus using multi gene sequence data. Vet Microbiol, 174, 39–49.

Kwiatek O., Ali Y.H., Saeed I.K., Khalafalla A.I., Mohamed O.I., Obeida A.A., Abdelrahman M.B., Osman H.M., Taha K.M., Abbas Z., El Harrak M., Lhor Y., Diallo A., Lancelot R., Albina E. & Libeau G. 2011. Asian lineage of peste des petits ruminants virus, Africa. Emerging Infect Dis, 17, 1223–1231.

Kwiatek O., Minet C., Grillet C., Hurard C., Carlsson E., Karimov B., Albina E., Diallo A. & Libeau G. 2007. Peste des petits ruminants (PPR) outbreak in Tajikistan. J Comp Pathol, 136, 111–119.

Lefèvre P.C. & Diallo A. 1990. Peste des petits ruminants. Rev Off Int Epizoot, 9, 935–981.

Libeau G., Diallo A. & Parida S. 2014. Evolutionary genetics underlying the spread of Peste des petits ruminants virus. Anim Front, 4, 14–20.

Luka P.D., Erume J., Mwiine F.N., Ayebazibwe C. & Shamaki D. 2011. Molecular characterization and phylogenetic study of peste des petits ruminants viruses from north central states of Nigeria. BMC Vet Res, 7, 32.

Mahapatra M., Sayalel K., Muniraju M., Eblate E., Fyumagwa R., Shilinde L., Mdaki M., Keyyu J., Parida S. & Kock R. 2015. Spillover of Peste des Petits Ruminants Virus from Domestic to Wild Ruminants in the Serengeti Ecosystem, Tanzania. Emerging Infect Dis, 21, 2230–2234.

Mantip S., Quan M., Shamaki D. & Van Vuuren M. 2016. Comparison of nucleotide sequences of recent and previous lineages of peste-des-petits-ruminants viruses of sheep and goats in Nigeria. Onderstepoort J Vet Res, 83, e1–e10.

Misinzo G., Kgotlele T., Muse E.A., Doorsselaere J.V., Berg M. & Munir M. 2015. Peste des petits ruminants virus lineage II and IV from goats in southern Tanzania during an outbreak in 2011. British J Virol, 2, 1-4.

Munir M., Zohari S., Suluku R., Leblanc N., Kanu S., Sankoh F.A., Berg M., Barrie M.L., Ståhl K. 2012. Genetic characterization of peste des petits ruminants virus, Sierra Leone. Emerging Infect Dis, 18, 193–195.

Obi T.U., Ojo M.O., Taylor W.P. & Rowe L.W. 1983. Studies on the epidemiology of Peste des petits ruminants in Southern Nigeria. Tropical Veterinarian, 1, 209-217.

Okoli I.C. 2003. Incidence and modulating effects of environmental factors on tripanosomosis, Peste des petits ruminants (PPR) and bronchopneumonia of West African dwarf goats in Imo state, Nigeria. Livestock Research for Rural Development, 15, 9–11.

Parida S., Muniraju M., Mahapatra M., Muthuchelvan D., Buczkowski H. & Banyard A.C. 2015. Peste des petits ruminants. Vet Microbiol, 181, 90–106.

Taylor W.P. & Abegunde A. 1979. The isolation of peste des petits ruminants virus from Nigerian sheep and goats. Res Vet Sci, 26, 94–96.

Thrusfield M. 2005. Veterinary epidemiology. 2nd Ed. Blackwell Science, Oxford, 117–198.

Tounkara K., Kwiatek O., Niang M., Abou Kounta Sidibe C., Sery A., Dakouo M., Salami H., Lo M.M., Ba A., Diop M., El Mamy A.B., El Arbi A.S., Barry Y., Isselmou E., Habiboullah H., Lella A.S., Doumbia B., Gueya M.B., Savadogo J., Ouattara L., Minougou G., Libeau G. & Bataille A. 2019. Genetic Evidence for Transboundary Circulation of Peste Des Petits Ruminants Across West Africa. Front Vet Sci, 6, 275.

Woma T.Y., Adombi C.M., Yu D., Qasim A.M.M., Sabi A.A., Maurice N.A., Olaiya O.D., Loitsch A., Bailey D., Shamaki D., Dundon W.G. & Quan M. 2016. Co-circulation of Peste-des-Petits-Ruminants Virus Asian lineage IV with Lineage II in Nigeria. Transbound Emerg Dis, 63, 235–242.

Wosu L.O. 1994. Current status of peste des petits ruminants (PPR) disease in small ruminants: a review article. Stud Res Vet Med, 2, 83–90.

Wosu L.O., Okiri J.E. & Enwezor P.A. 1990. Optimal time for vaccination against Peste des petits ruminants (PPR) disease in goats in humid tropical zone in southern Nigeria. Arch Roum Pathol Exp Microbiol, 49, 283–291.

Zahur A.B., Ullah A., Hussain M., Irshad H., Hameed A., Jahangir M., Farooq M.S. 2011. Sero-epidemiology of peste des petits ruminants (PPR) in Pakistan. Prev Vet Med, 102, 87–92.

Zhou X.Y., Wang Y., Zhu J., Miao Q.-H., Zhu L.Q., Zhan S.H., Wang G.J. & Liu G.Q. 2018. First report of peste des petits ruminants virus lineage II in Hydropotes inermis, China. Transbound Emerg Dis, 65, e205–e209.

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Published

2021-12-31

How to Cite

Chukwudi, I. C. ., Mira, F., Ogbu, K. I., Malesa, R. P., Purpari, G., Luka, P. D., Ugochukwu, E. I. ., Heath, L. E., Guercio, A., & Chah, K. F. (2021). Peste des petits ruminants in Nigeria: identification and variations of lineage II and lineage IV in sheep and goats. Veterinaria Italiana, 57(3). https://doi.org/10.12834/VetIt.2390.14842.1

Issue

Vol. 57 No. 3 (2021)

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