Detection and dynamics of tumor necrosis factor alpha in the diagnosis and treatment of canine heartworm disease
DOI:
https://doi.org/10.12834/VetIt.2662.22847.2Keywords:
Dogs, Heartworm disease, Tumor necrosis factor alphaAbstract
The aim of this study was to determine the concentration of TNF-alpha (TNF-α) in dogs naturally infected with Dirofilaria immitis (D. immitis) and to assess whether there are any changes in TNF-α concentration and their dependence during therapy for heartworm disease (HWD). For this study, 14 client-owned dogs with HWD were selected. Clinical and parasitological examinations (modified Knott test for circulating microfilariae and SNAP Test IDEXX for circulating D. immitis antigen) had been used for diagnosing D. immitis and HWD. All dogs were treated with an alternative therapy for HWD (oral doxycycline 10 mg/kg b.w., once daily for 6 weeks, then alternately 4 weeks without and 2 weeks with the medication, and oral ivermectin 6-14 µg/kg b.w., every 2 weeks). The dogs blood sera at the moment of HWD diagnosis, during and at the end of therapy were frozen for further quantifying of TNF-α (Canine TNF-alpha ELISA kit, Thermo scientific). At the moment of HWD diagnosis TNF-α was detected in 9 dogs (7.21±12.44 pg/ml). Concentration of TNF-α was not significantly change during the therapy, neither related to the level of D. immitis antigen nor to antigen level changes. The alternative therapy for HWD has no influence on TNF-α concentration dynamics.References
AHS, American heartworm society. Current canine guidelines for the prevention, diagnosis and management of heartworm infection in dogs. 2020. Available from: https://d3ft8sckhnqim2.cloudfront.net/images/pdf/2020_AHS_Canine_Guidelines.pdf?1580934824.
Akasaka T., Yoshida K., Hozumi T., Takagi T., Kaji S., Kawamoto T., Ueda Y., Okada Y., Morioka S. & Yoshikawa J. 1998. Restricted coronary flow reserve in patients with mitral regurgitation improves after mitral reconstructive surgery. J Am Coll Cardiol, 32, 1923-1930.
Anguita M., Arion J.M., Bueno G., Latre J.M., Sancho M., Torres F., Gimmenez D., Concha M. & Vallas F. 1993. Clinical and hemodynamic predictors of survival in patients aged-65 years with severe congestive heart failure secondary to ischemic or non-ischemic dilated heart failure. Am J Cardiol, 72(5), 413-417.
Baisan R.A., De Rosa A., Di Loria A., Vulpe V. & Piantedosi D. 2016. Cardiac biomarkers in clinical practice of dog and cat – a review. HVM Bioflux, 8 (1), 50-55.
Bastien B.C., Patil A. & Satyaraj E. 2015. The impact of weight loss on circulating cytokines in Beagle dogs. Vet Immunol Immunopathol,163(3), 174-182.
Bazzocchi C., Mortarino M., Grandi G., Kramer L.H., Genchi C., Bandi C., Genchi M., Sacchi L. & McCall J.W. 2008. Combined ivermectin and doxycycline treatment has microfilaricidal and adulticidal activity against Dirofilaria immitis in experimantally infected dogs. Int J Parasitol, 38, 1401-1410.
Carretón E., Corbera J. A., Juste M. C., Morchón R., Simón F. & Montoya-Alonso J. A. 2011. Dirofilaria immitis infection in dogs: cardiopulmonary biomarker levels. Vet Parasitol, 176, 313-316.
Carretón E., Morchón R. & Montoya-Alonso J. A. 2017. Cardiopulmonary and inflammatory biomarkers in heartworm disease. Parasites & Vectors, 10 (Suppl 2):534, November, 10.1186/s13071-017-2448-2.
Carswell E.A., Old L.J., Kassel R.L., Green S. & Fiore N., Williamson B. 1975. An endotoxin-induced serum factor that causes necrosis of tumors. Proc Natl Acad Sci U S A., 72(9), 3666–3670.
Doyama K., Fujiwara H., Fukumoto M., Tanaka M., Fujiwara Y., Oda T., Inada T., Ohtani S., Hasegawa K., Fujiwara T. & Sasayama S. 1996. Tumour necrosis factor is expressed in cardiac tissues of patients with heart failure. Int J Cardiol,54(3), 217–225.
Dunn J.K. 2000. Diseases of the cardiovascular system. In Textbook of small animal medicine (J.K. Dunn, ed.). W. B. Saunders, London, 255-344.
ESDA, European Society of Dirofilariosis and Angiostrongylosis. Guidelines for Clinical Management of Canine Heartworm Disease. 2017. Available from: http://www.esda.vet.
Fann J.I., Ingels N.B. & Miller D.C. 1997. Pathophysiology of mitral valve disease and operative indications. In Cardiac Surgery in the Adult (L. H. Edmunds, ed). McGraw-Hill, New York, 959-990.
Frank L., Mann S. & Levine C.B., Cummings B.P. & Wakshlag J.J. 2015. Increasing body condition score is positively associated interleukin-6 and monocyte chemoattractant protein-1 in Labrador retrievers. Vet Immunol Immunopathol, 167(3–4), 104–109.
Genchi G., Venco L. & Genchi M. 2007. Guideline for the laboratory diagnosis of canine and feline Dirofilaria infections. In Mappe Parassitologighe. 8 Dirofilaria. (C. Genchi, L. Rinaldi, G. Cringoli, eds.), Veterinary Parasitology and Parasitic Disease, Naples, 137-145.
Genchi C., Kramer L.H., Sassera D., Bandi C. 2012. Wolbachia and Its Implications for the Immunopathology of Filariasis. Endocrin Metab Immune Disord Drug Targets, 12, 53-56.
Keith J.C., Schaub R.G. & Ralings C.1983. Early arterial injury-induces myointimal proliferation in canine pulmonary artery. Am J Vet Res, 44, 181-186.
Kilpatrick S, Gow AG, Foale RD, Tappin S.W., Carruthers H., Reed N., Yool D.A., Woods S., Marques A.I., Jalan R. & Mellanby R.J. 2014. Plasma cytokine concentrations in dogs with a congenital portosystemic shunt. Vet J, 200(1), 197–199.
Kim H.-S., Kang J.-H., Jeung E.-B. & Yang M.-P. 2016. Serum Concentrations of Leptin and Adiponectin in Dogs with Myxomatous Mitral Valve Disease. J Vet Intern Med, 30, 1589–1600.
Kitagawa H., Kitoh K., Inoue H., Ohba Y., Suzuki F. & Sasaki Y. 2000. Plasma rennin activities, angiotensin II concentrations, atrial natriuretic peptide concentrations and cardiopulmonary function values in dogs with severe heartworm disease. JVMS, 62(4), 453–455.
Kittleson M.D. 1998. Heartorm Infestation and Disease (Dirofilariasis). In Small Animal Cardiovascular Disease (M.D. Kittleson, Kienle R.D., eds.). Mosby, St Louis, 370-401.
Knight D.H. 1980. Evolution of pulmonary artery disease in canine dirofilariasis: evaluation by blood pressure measurements and angiography. In Heartworm Symposium 1980 (G.F. Otto, ed.). Proc. the Heartorm Symposium, Veterinary Medicine Publishing, Bonner Springs, Kansas, 55-62.
Mann D.L., Deswal A., Bozkurt B. & Torre-Amione G. 2002. New therapeutics for chronic heart failure. Annu Rev Med,53, 59–74.
McCall J.W., Genchi C., Kramer L., Guerrero J., Venco L.2008. Heartworm Disease in Animals and Humans. Adv Parasitol, 66, 193-285.
Morchón R., Simón F., Gonzalez-Miguel J., Mellado I. 2009. Relationship Dirofilaria/hosts: cellular and molecular mechanisms of the heartworm disease vascular pathology. Proceedings Second European Dirofilaria days (SEDD), 124-133, 16th-18th September, Salamanca, Spain.
Neuhaus T., Totzke G., Gruenewald E., Juesten H.P., Sachinidis A., Vetter H. & Ko Y. 2000. Tumour necrosis factor-alpha gene expression and production in human umbilical arterial endothelial cells. Clin Sci, 98(4), 461–470.
Nielsen L.N., Kjelgaard-Hansen M. & Kristensen A.T. 2013. Monocyte chemotactic protein-1 and other inflammatory parameters in Bernese Mountain dogs with disseminated histiocytic sarcoma. Vet J, 198(2), 424–428.
O’Neill S., Drobatz K., Satyaraj E. & Hess R. 2012. Evaluation of cytokines and hormones in dogs before and after treatment of diabetic ketoacidosis and in uncomplicated diabetes mellitus. Vet Immunol Immunopathol,148(3), 276–283.
Pagani F.D., Baker L.S., Hsi C., Knox M., Fink M.P. & Visner M.S. 1992. Left ventricular systolic and diastolic dysfunction after infusion of tumor necrosis factor-α in conscious dogs. J Clin Invest ,90, 389-398.
Richter K.R., Amirah N. Nasr A.N. & Mexas A.M. 2018. Cytokine Concentrations Measured by Multiplex Assays in Canine Peripheral Blood Samples. Vet Pathol, 55(1), 53-67.
Schaub R.G., Rawlings C.A. & Keith J.C. 1981. 1981. Pletelet adhesion and myointinal proliferation in canine pulmonary arteries. Am J Pathol, 104, 13-22.
Simón F., Kramer L.H., Román A, Blasini W., Morchon R., Marcos-Atxutegi C., Grandi G., Genchi C. 2007. Immunopathology of Dirofilaria immitis Infection. Vet Res Commun 31, 161–171.
Simón F, Siles-Lucas M, Morchón R, González-Miguel J, Mellado I, Carretón E, Montoya-Alonso JA. 2012. Human and animal dirofilariasis: the emergence of a zoonotic mosaic. Clin Microbiol Rev, 25(3), 507-544.
Smolens I.A., Pagani F.D. & Bolling S.F. 2000. Mitral valve repair in heart failure. Eur J Heart Fail, 2, 365-371.
Takemura N., Koyama H., Sako T., Ando K., Motoyoshi S. & Marumo F. 1991. Plasma atrial natriuretic peptide (ANP) in dogs with mild heartworm infection. JVMS, 53(2), 323–325.
Uchide T. & Saida K. 2005. Elevated endothelin-1 expression in dogs with heartworm disease. Journal of Veterainary Medical Science, 67(11), 1155–1161.
Urschel K. & Cicha I. 2015. TNF-α in the cardiovascular system: from physiology to therapy. Int J Interfern Cytokine Mediat Res,7, 9–25.
Venco L. 2009. New insight into HW disease management: from old legends to the present looking to the future. Proceedings Second European Dirofilaria days (SEDD), 107-115, 16th-18th September, Salamanca, Spain.
Venco L., Bertazzolo W., Giordano G. & Paltrinieri S. 2014. Evaluation of C-reactive protein as a clinical biomarker in naturally heartworm-infected dogs: a field study. Vet Parasitol, 206(1–2), 48–54.
von Asmuth E.J., Dentener M.A., Ceska M. & Buurman W.A. 1994. IL-6, IL-8 and TNF production by cytokine and lipopolysaccharide-stimulated human renal cortical epithelial cells in vitro. Eur Cytokine Netw, 5(3), 301–310.
von Pfeil D.J., Cummings B.P., Loftus J.P., Levine C.B., Mann S., Doney R.L., Griffitts C. & Akshlag J.J. 2015. Evaluation of plasma inflammatory cytokine concentrations in racing sled dogs. Can Vet J;56(12), 1252–1256.
Ware W. 2011. Heartworm disease. In Cardiovascular disease in small animal disease (W. Ware, ed.). Manson Publishing/The veterinary press, London, 351-371.
Warner S.J. & Libby P. 1989. Human vascular smooth muscle cells. Target for and source of tumor necrosis factor. J Immunol, 142(1), 100–109.
Yoon W.-K., Kim Y.-W., Suh S.I.L., Choi R., Lee S.-G. & Hyun C. 2017. Evaluation of cardiopulmonary and inflammatory markers in dogs with heartworm infection during treatment with the 2014 American Heartworm Society recommended treatment protocol. Parasit Vectors, 10(Suppl 2), 535, 9 November 2017, 10.1186/s13071-017-2427-7.
Zygner W., Gójska-Zygner O., Bąska P. & Długosz E. 2014. Increased concentration of serum TNF alpha and its correlations with arterial blood pressure and indices of renal damage in dogs infected with Babesia canis. Parasitol Res, 113, 1499–1503.
