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Experimental infection of cattle, sheep, and goats with the newly emerged epizootic hemorrhagic disease virus serotype 8
Vol. 59 No. 4 (2023), Paper
Vol. 59 No. 4 (2023)

Experimental infection of cattle, sheep, and goats with the newly emerged epizootic hemorrhagic disease virus serotype 8

Paper
https://doi.org/10.12834/VetIt.3433.23112.1
Published 2023-12-20
  • Massimo Spedicato+−
  • Francesca Profeta+−
  • Sarah Thabet+−
  • Liana Teodori
  • Alessandra Leone
  • Ottavio Portanti
  • Maura Pisciella
  • Barbara Bonfini
  • Simone Pulsoni
  • Francesca Rosso
  • Emanuela Rossi
  • Paola Ripà
  • Angela De Rosa
  • Eugenia Ciarrocchi
  • Roberta Irelli
  • Antonio Cocco
  • Corinne Sailleu
  • Nicola Ferri
  • Tiziana Di Febo
  • Damien Vitour
  • Emmanuel Breard
  • Daniele Giansante
  • Soufien Sghaier
  • Thameur Ben Hassine
  • Stephan Zientara
  • Romolo Salini
  • Salah Hammami
  • Giovanni Savini
  • Alessio Lorusso+−
Massimo Spedicato
Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise, Campo Boario, 64100 Teramo-Italy
Francesca Profeta
Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise, Campo Boario, 64100 Teramo-Italy
Sarah Thabet
Service de Microbiologie, Immunologie et Pathologie Générale, École Nationale de Médecine Vétérinaire de Sidi Thabet, IRESA, Université de la Manouba, Tunis 2020, Tunisia
Liana Teodori
Alessandra Leone
Ottavio Portanti
Maura Pisciella
Barbara Bonfini
Simone Pulsoni
Francesca Rosso
Emanuela Rossi
Paola Ripà
Angela De Rosa
Eugenia Ciarrocchi
Roberta Irelli
Antonio Cocco
Corinne Sailleu
Nicola Ferri
Tiziana Di Febo
Damien Vitour
Emmanuel Breard
Daniele Giansante
Soufien Sghaier
Thameur Ben Hassine
Stephan Zientara
Romolo Salini
Salah Hammami
Giovanni Savini
Alessio Lorusso
IZS Abruzzo e Molise "G. Caporale", Virology Unit
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Keywords

EHDV
cattle
sheep
goats

How to Cite

Spedicato, M., Profeta, F., Thabet, S., Teodori, L., Leone, A., Portanti, O., Pisciella, M., Bonfini, B., Pulsoni, S., Rosso, F., Rossi, E., Ripà, P., De Rosa, A., Ciarrocchi, E., Irelli, R., Cocco, A., Sailleu, C., Ferri, N., Di Febo, T., Vitour, D., Breard, E., Giansante, D., Sghaier, S., Ben Hassine, T. ., Zientara, S., Salini, R., Hammami, S., Savini, G., & Lorusso, A. (2023). Experimental infection of cattle, sheep, and goats with the newly emerged epizootic hemorrhagic disease virus serotype 8. Veterinaria Italiana, 59(4). https://doi.org/10.12834/VetIt.3433.23112.1
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Funding data

  • Ministero dell'Università e della Ricerca
    Grant numbers A novel integrated and sustainable approach to monitor and control Bluetongue spreadin the Mediterranean Basin (Blue-Med)
  • Ministero della Salute
    Grant numbers Studio dei principali aspetti epidemiologici, clinico‑ patologici e virologici di EHD al fine di valutarne l’evoluzione e supportare la early detection attraverso adeguati piani di sorveglianza"

Abstract

Epizootic hemorrhagic disease virus serotype 8 (EHDV-8) emerged in Europe for the first time in late 2022. In this study, we investigated the kinetics of EHDV-8 infection in cattle, sheep, and goats.  Following experimental infection with EHDV-8, four out of five calves displayed fever, while another calf exhibited ulcerative and crusty lesions of the muzzle. RNAemia peaked at day 7 post infection in all calves and remained relatively stable till the end of the study, at 78 days post infection. Infectious virus was isolated up to 21 days post infection in one calf. As far as small ruminants are concerned, one sheep experienced fever and two out of five had consistent RNAemia that lasted until the end of the study. Remarkably, infectious virus was evidenced at day 7 post infection in one sheep. In goats, no RNA was observed. All infected animals seroconverted, and a neutralizing immune response was observed in all species, with calves exhibiting a more robust response than sheep and goats.

Our study provides insights into the kinetics of EHDV-8 infection and the host immune responses. We also highlight that sheep may also play a role in EHDV-8 epidemiology. Altogether, the data gathered in this study could have important implications for disease control and prevention strategies, providing crucial information to policy makers to mitigate the impact of this viral disease on livestock.

https://doi.org/10.12834/VetIt.3433.23112.1
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Copyright (c) 2020 Massimo Spedicato, Francesca Profeta, Sarah Thabet, Liana Teodori, Alessandra Leone, Ottavio Portanti, Maura Pisciella, Barbara Bonfini, Simone Pulsoni, Francesca Rosso, Emanuela Rossi, Paola Ripà, Angela De Rosa, Eugenia Ciarrocchi, Roberta Irelli, Antonio Cocco, Corinne Sailleu, Nicola Ferri, Tiziana Di Febo, Damien Vitour, Emmanuel Breard, Daniele Giansante, Soufien Sghaier, Thameur Ben Hassine, Stephan Zientara, Romolo Salini, Salah Hammami, Giovanni Savini, Alessio Lorusso

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