Multispacer sequence typing of Coxiella burnetii from milk and hard tick samples from ruminant farms in Lebanon

Authors

  • Mayssaa Fawaz Dabaja EDST Lebanese University Beirut-Lebanon
  • Grazia Greco Faculty of Veterinary Medicine, University of Bari, Bari, Italy
  • Valeria Blanda Istituto Zooprofilattico Sperimentale della Sicilia “A. Mirri”, Italy
  • Maria Tempesta Faculty of Veterinary Medicine, University of Bari, Bari, Italy
  • Ali Bayan Lebanese University, Faculty of Sciences, Section I, Hadath, Lebanon
  • Alessandra Torina Istituto Zooprofilattico Sperimentale della Sicilia “A. Mirri”, Italy
  • Gesualdo Vesco Istituto Zooprofilattico Sperimentale della Sicilia “A. Mirri”, Italy
  • Rosalia D'Agostino Istituto Zooprofilattico Sperimentale della Sicilia “A. Mirri”, Italy
  • Rossella Lelli Istituto Zooprofilattico Sperimentale dell' Abruzzo e del Molise, Teramo, Italy
  • Mohamad Ezzedine 2Lebanese University, Doctoral School of sciences and Technology, Beirut, Lebanon
  • Hussein Mortada Lebanese University, Faculty of Agriculture, Beirut, Lebanon
  • Didier Raoult URMITE, UM 63, CNRS 7278, IRD 198, Inserm 1095, Aix-Marseille University, Marseille, France
  • Pierre Edouard Fournier URMITE, UM 63, CNRS 7278, IRD 198, Inserm 1095, Aix-Marseille University, Marseille, France
  • Mohamad Mortada Lebanese University, Doctoral School of sciences and Technology, Beirut, Lebanon

DOI:

https://doi.org/10.12834/VetIt.1799.13290.1

Keywords:

Coxiella burnetii, MST, Lebanon, Ticks, Milk

Abstract

his study was carried out to detect and characterize Coxiella burnetii in ruminant milk samples and in different tick species from seropositive farms in four Lebanese regions. Milk and tick samples were screened for C. burnetii presence by quantitative real-time PCR (qPCR) targeting IS1111 region followed by multispacer sequence typing (MST). The overall positive percentages of 9.6% (27/282) and 95.45% (84/88) for C. burnetii were recorded in ruminant milk and tick samples, respectively. In detail, the C. burnetii DNA was recorded in 52/54 (96.3%) of Rhipicephalus annulatus, 20/21 (95.24%) of Rhipicephalus turanicus, 6/6 (100%) of Hyalomma anatolicum, 5/6 (83.3%) of Rhipicephalus sanguineus and 1/1 of Rhipicephalus bursa. After genotyping of some IS1111-positive samples (17/111), different MST genotypes were identified. Out of 15 positive ticks, 10 were infected with MST2 genotype, 4 were infected with MST7 genotype and 1 was infected with MST57. Moreover, genotypes MST20 and MST58 were found in one cow and one goat milk samples, respectively. The present study confirmed the high genetic diversity of C. burnetii in Lebanon.

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Published

2020-12-01

How to Cite

Dabaja, M. F., Greco, G., Blanda, V., Tempesta, M., Bayan, A., Torina, A., Vesco, G., D’Agostino, R., Lelli, R., Ezzedine, M., Mortada, H., Raoult, D., Fournier, P. E., & Mortada, M. (2020). Multispacer sequence typing of Coxiella burnetii from milk and hard tick samples from ruminant farms in Lebanon. Veterinaria Italiana, 56(4), 289–296. https://doi.org/10.12834/VetIt.1799.13290.1

Issue

Vol. 56 No. 4 (2020)

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