Relationship between gastrointestinal nematode burden, blood parameters and hair cortisol in Italian local sheep not treated with anthelmintics
DOI:
https://doi.org/10.12834/VetIt.3584.27392.1Keywords:
Sheep, Zerasca, Gastrointestinal parasite, Blood parametersAbstract
This study explored the interplay between gastrointestinal nematode burden, blood parameters, and hair cortisol in Zerasca sheep, a local breed of Northeastern Tuscany (Italy). Moreover, the correlation between BCS and Eggs per Gram (EPG) was analyzed to verify the possible parasite impact. The study involved twelve ewes sampled for faeces, blood and wool three times, in the morning, from August 2022 to March 2023. Complete blood count, alanina-aminotransferase, aspartate-aminotransferase, nitrogen ureic, beta-hydroxybutyric acid, non-esterified fatty acids, total proteins, creatinine, calcium, chloride, potassium, and oxygen free radicals were analyzed. Fresh faecal samples were collected and subjected to the Mac Master technique for EPG. Wool was collected to quantify hair cortisol. BCS was recorded using a 1-5 scale. The ANOVA test was performed to detect differences in metabolic and immunological parameters, as well as hair cortisol levels, in relation to EPG levels. Non parametric correlation between EPG and BCS was conducted. Only red blood cells, haematocrit, mean corpuscular haemoglobin concentration, and calcium were significantly associated with EPG levels. Some parameters were outside the normal range. The correlation between BCS and EPG resulted negative and statistically significant. These results showed an overall well-being of the tested animals within this specific contextReferences
Ambrosi, M. Parassitologia Zootecnica. 1995. Edagricole.
Baihaqi, Z.A., Nurcahyo, W. & Widiyono, I. 2020. Prevalence naturally infected gastrointestinal parasites and complete blood count condition on Wonosobo sheep at Wonosobo District, Central Java, Indonesia. Biodiversitas, 21, 7, 3057-3061. DOI: 10.13057/biodiv/d210724.
Bello, A.A. & Tsado, D.N. 2014. Quality and sensory evaluation of meat from Yankasa rams fed sorghum stover supplemented with varying levels of dried poultry droppings based diet. Int J Agric Food Sci, 2, 1-8.
Benvenuti, M.N., Pisseri, F., Goracci, J., Giuliotti, L., Macchioni, F., Verità, P. & Guidi, G. 2011. Use of homeopathy in parasite control in a flock of Zerasca sheep. In New trends for innovation in the Mediterranean animal production, EAAP 129, 296-300.
Benvenuti, N., Giuliotti, L., Goracci, J. & Verità, P. 2006. Study of gastrointestinal parasite dynamics in Zerasca sheep aimed at reducing anthelmintic treatment. In Animal products from the Mediterranean area, EAAP 131, 283-287. DOI: https://doi.org/10.3920/9789086865680_034.
Bernabucci, U., Ronchi, B., Lacetera, N. & Nardone, A. 2005. Influence of body condition score on relationships between metabolic status and oxidative stress in periparturient dairy cows. J Diary Sci, 88(6), 2017-2026.
Burnard, C., Ralph, C., Hynd, P., Edwards, J.H. & Tilbrook, A. 2016. Hair cortisol and its potential value as a physiological measure of stress response in human and non-human animals. Anim Prod Sci, 57(3), 401-414.
Calin, S., Codreanu, I., Iacobescu, M. & Codreanu, M.D. 2020. Correlations between different physiological stages and the prevalence of calcium metabolism disorders in ewes. Lucrari Stiintifice-Universitatea de Stiinte Agricole a Banatului Timisoara, Medicina Veterinara, 53(2), 23-29.
Dwyer, C.M. & Bornett, H.L.I. 2004. Chronic stress in sheep: assessment tools and their use in different management conditions. Anim Welf, 13(3), 293-304.
Ebrahim, Z.K. 2018. Effect of Gastrointestinal Parasites Infestation on Some Hematological and Biochemical Parameters in Sheep. Alex J Vet Sci, 59(1), 44-47.
FAO. The State of the World’s Biodiversity for Food and Agriculture. In FAO Commission on Genetic Resources for Food and Agriculture Assessments. Bélanger, J., Pilling, D., Eds., FAO: Rome, Italy, 2019. Available online: http://www.fao.org/3/CA3129EN/CA3129EN.pdf.
Fell, L.R., Lynch, J.J., Adams, D.B., Hinch, G.N., Munro, R.K. & Davies, H.I. 1991. Behavioural and physiological effects in sheep of a chronic stressor and a parasite challenge. Aust J Agric Res, 42(8), 1335-1346.
Fürtbauer, I., Solman, C. & Fry, A. 2019. Sheep wool cortisol as a retrospective measure of long-term HPA axis activity and its links to body mass. Domest Animal Endocrinol, 68, 39-46.
Goddard, P., Waterhouse, T., Dwyer, C. & Stott, A. 2006. The perception of the welfare of sheep in extensive systems. Small Rumin Res, 62(3), 215-225.
Kenyon, F. & Jackson, F. 2012. Targeted flock/herd and individual ruminant treatment approaches. Vet Parasitol, 186, 10-17.
Kozat, S., Yüksek, N., Göz, Y. & Keleş, İ. 2006. Serum iron, total iron-binding capacity, unbound iron-binding capacity, transferrin saturation, serum copper, and hematological parameters in pregnant Akkaraman ewes infected with gastro-intestinal parasites. Turk J Vet Anim Sci, 30(6), 601-604.
Lykkesfeldt, J. & Svendsen, O. 2007. Oxidants and antioxidants in disease: oxidative stress in farm animals. Vet J, 173(3), 502-511.
Mariti, C., Diverio, S., Gutiérrez, J. Baragli, P. & Gazzano, A. 2020. Partial analytic validation of determination of cortisol in dog hair using a commercial EIA kit. Dog Behave, 6(3): 1-15.
Masters, D.G. 2018 Practical implications of mineral and vitamin imbalance in grazing sheep. Anim Prod Sci, 58(8), 1438-1450.
Nazifi, S., Ghafari, N., Farshneshani, F., Rahsepar, M. & Razavi S.M. 2010. Reference Values of Oxidative Stress Parameters in Adult Iranian Fat-Tailed Sheep. Pakistan Vet J, 30(1), 13-16.
Ngetich, E.C.V., Ngeiywa, M. & Ng’Wena, G.M. 2019. Haematological parameters by age and sex of asymptomatic indigenous cattle and sheep infected with gastrointestinal parasites in Kerio Valley, Kenya. Am J Biomed Res, 7(2), 44-50. DOI: 10.12691/ajbr-7-2-4.
Oramari, R.A., Bamerny, A.O. & Zebari, H.M. 2014. Factors affecting some hematology and serum biochemical parameters in three indigenous sheep breeds. Adv Environ Sci Technol, 21(12), 56-62.
Paddock, R. 2011. Haemonchus contortus in sheep and goats: An insidious killer. Indiana Animal Disease Diagnostic Laboratory Fall.
Permin, A. & Hansen, J. 1998. Epidemiology. Diagnosis and control of poultry parasites. FAO Animal Health Manual.
Radkowska, I. & Herbut, E. 2014. Hematological and biochemical blood parameters in dairy cows depending on the management system. Anim Sci Pap Rep, 32(4), 685-695.
Rathwa, D., Vasava, A.A., Pathan, M.M., Madhira, S.P., Patel, Y.G. & Pande, A.M. 2017. Effect of season on physiological, biochemical, hormonal, and oxidative stress parameters of indigenous sheep Sawankuma. Vet. World, EISSN: 2231-0916 Available at www.veterinaryworld.org/Vol.10/June-2017/13.
Russel, A.J.F., Doney, J.M. & Gunn, R.G. 1969. Subjective assessment of body fat in live sheep. J Agr Sci, 72, 451-454.
SAS, JMP. User’s Guide, version 5.0, SAS Inst.: Cary, NC, USA, 2002.
Sotiraki, S., Stefanakis, A., Hoste, H., Mauer, V., Butler, G. & Leifert, C. 2013. The role of biotic and abiotic stress factors on sheep welfare: The example of parasites and climatic changes in European countries. In Feeding and management strategies to improve livestock productivity, welfare and product quality under climate change, Zaragoza, Spain, 2013, Lopez-Francos A. (ed.). CIHEAM INRAT OEP IRESA FAO, 201, 159-169.
Soto-Barrientos, N., Chan-Pérez, J.I., España-España, E., Novelo-Chi, L.K., Palma-Ávila, I., Ceballos-Mendoza, A.C., Sarabia-Hernández, J.A., Santos-Ricalde, R.H., Cámara-Sarmiento, R. & Torres-Acosta J.F.J. 2018. Comparing body condition score and FAMACHA© to identify hair-sheep ewes with high faecal egg counts of gastrointestinal nematodes in farms under hot tropical conditions. Small Rumin Res, 167, 92-99.
Theodoropoulos, G., Theodoropoulos, H., Zervas, G. & Bartziokas, E. 2000. Nematode parasite control practices of sheep and goat farmers in the region of Trikala, Greece. J Helminthol, 74, 89-93.
Tüfekci, H. & Sejian, V. 2023. Stress factors and their effects on productivity in sheep. Animals, 13(17), 2769.
Vijay, P., Khajuriya, J.K., Soodan, J.S., Neelesh, S., Upadhyaya, S.R. & Rajesh, K. 2010. Influence of gastrointestinal parasites on certain blood components of sheep. Indian J Small Rumin, 16(1), 134-136.
Weavera, S.J., Hynda, P.I., Ralpha, C.R.J.E., Hocking Edwards, J.E., Burnard, C.L., Narayan, E. & Tilbrook A.J. 2021. Measures of stress, particularly Chronic elevation of plasma cortisol causes differential expression of predominating glucocorticoid in plasma, saliva, faecal, and wool matrices in sheep. Domest Anim Endocrinol, 74, 106503.
