First molecular identification of the zoonotic parasite Anisakis pegreffii (Nematoda: Anisakidae) in Albania

Authors

DOI:

https://doi.org/10.12834/VetIt.3855.37828.2

Keywords:

Anisakis pegreffii, Ionian Sea, Albanian coast, Atlantic horse mackerel, food safety, molecular identification

Abstract

For the first time, Anisakidae larvae in commercially caught fish from the southwestern Ionian Sea off the Albanian coast were identified using molecular techniques. Atlantic horse mackerel (Trachurus trachurus) from the Vlora district were examined for parasitic infections. Enzymatic digestion revealed the presence of Anisakis spp. larvae, which were subsequently analysed at ISS Rome by multiplex PCR and PCR-RFLP. Molecular results confirmed the larvae as Anisakis pegreffii. This study provides the first molecular confirmation of Anisakis species in Albanian waters. The detection of Anisakis larvae highlights potential risks for seafood safety and public health, underlining the importance of regular monitoring and risk assessment in the region.

Author Biographies

Ani Vodica, Food Safety and Veterinary Institute

Ani VodicaHead of Diagnosis Section Animal Health Department Food Safety and Veterinary Institute   St"Aleksander Moisiu", Nr.82 Tirana, Albania (E): Ani.Vodica@isuv.gov.al| (W): www.isuv.gov.al

Marco Lalle, Istituto Superiore di Sanità

Department of Infectious Diseases Unit of Foodborne and Neglected Parasitic Diseases, European Union Reference Laboratory for Parasites, viale Regina Elena 299, 00161 Roma, Italy

Federica Santolamazza, Istituto Superiore di Sanità

Istituto Superiore di Sanità, Department of Infectious Diseases Unit of Foodborne and Neglected Parasitic Diseases, European Union Reference Laboratory for Parasites, viale Regina Elena 299, 00161 Roma, Italy  

References

Abollo, E., Gestal, C., & Pascual, S. (2001). Anisakis infestation in marine fish and cephalopods from Galician waters: an updated perspective. Parasitology research, 87(6), 492–499. https://doi.org/10.1007/s004360100389.

Audicana, M. T., & Kennedy, M. W. (2008). Anisakis simplex: from obscure infectious worm to inducer of immune hypersensitivity. Clinical microbiology reviews, 21(2), 360–379. https://doi.org/10.1128/CMR.00012-07.

Berland, B. (1961). Nematodes from some Norwegian marine fishes. Sarsia, 2, 1–50.

Brokaj, M. (2014). The Impact of the Gastronomic Offer in Choosing Tourism Destination: The Case of Albania. Academic Journal of Interdisciplinary Studies, 3(2), 249–258. DOI: 10.5901/ajis.2014.v3n2p249.

Broglia, A., & Kapel, C. (2011). Changing dietary habits in a changing world: emerging drivers for the transmission of foodborne parasitic zoonoses. Veterinary parasitology, 182(1), 2–13. https://doi.org/10.1016/j.vetpar.2011.07.011.

Cavallero, S., Ligas, A., Bruschi, F., & D'Amelio, S. (2012). Molecular identification of Anisakis spp. from fishes collected in the Tyrrhenian Sea (NW Mediterranean). Veterinary parasitology, 187(3-4), 563–566. https://doi.org/10.1016/j.vetpar.2012.01.033.

Chai, J. Y., Darwin Murrell, K., & Lymbery, A. J. (2005). Fish-borne parasitic zoonoses: status and issues. International journal for parasitology, 35(11-12), 1233–1254. https://doi.org/10.1016/j.ijpara.2005.07.013.

Chaligiannis, I., Lalle, M., Pozio, E., & Sotiraki, S. (2012). Anisakidae infection in fish of the Aegean Sea. Veterinary parasitology, 184(2-4), 362–366. https://doi.org/10.1016/j.vetpar.2011.09.007.

Cipriani, P., Smaldone, G., Acerra, V., D'Angelo, L., Anastasio, A., Bellisario, B., Palma, G., Nascetti, G., & Mattiucci, S. (2015). Genetic identification and distribution of the parasitic larvae of Anisakis pegreffii and Anisakis simplex (s. s.) in European hake Merluccius merluccius from the Tyrrhenian Sea and Spanish Atlantic coast: implications for food safety. International journal of food microbiology, 198, 1–8. https://doi.org/10.1016/j.ijfoodmicro.2014.11.019.

Cipriani, P., Sbaraglia, G. L., Paoletti, M., Giulietti, L., Bellisario, B., Palomba, M., Bušelić, I., Mladineo, I., Nascetti, G., & Mattiucci, S. (2018). The Mediterranean European hake, Merluccius merluccius: Detecting drivers influencing the Anisakis spp. larvae distribution. Fisheries Research, 202, 79–89. https://doi.org/10.1016/j.fishres.2017.07.010.

Costa, A., Cammilleri, G., Graci, S., Buscemi, M. D., Vazzana, M., Principato, D., Giangrosso, G., & Ferrantelli, V. (2016). Survey on the presence of A. simplex s.s. and A. pegreffii hybrid forms in Central-Western Mediterranean Sea. Parasitology international, 65(6 Pt A), 696–701. https://doi.org/10.1016/j.parint.2016.08.004.

D'Amelio, S., Mathiopoulos, K. D., Santos, C. P., Pugachev, O. N., Webb, S. C., Picanço, M., & Paggi, L. (2000). Genetic markers in ribosomal DNA for the identification of members of the genus Anisakis (Nematoda: ascaridoidea) defined by polymerase-chain-reaction-based restriction fragment length polymorphism. International journal for parasitology, 30(2), 223–226. https://doi.org/10.1016/s0020-7519(99)00178-2.

Debenedetti, Á. L., Madrid, E., Trelis, M., Codes, F. J., Gil‑Gómez, F., Sáez‑Durán, S., & Fuentes, M. V. (2019). Prevalence and risk of anisakid larvae in fresh fish frequently consumed in Spain: An overview. Fishes, 4(1), 13. https://doi.org/10.3390/fishes4010013.

Farjallah, S., Slimane, B. B., Busi, M., Paggi, L., Amor, N., Blel, H., Said, K., & D'Amelio, S. (2008). Occurrence and molecular identification of Anisakis spp. from the North African coasts of Mediterranean Sea. Parasitology research, 102(3), 371–379. https://doi.org/10.1007/s00436-007-0771-9.

Ishikura, H., & Namiki, M. (1989). Gastric anisakiasis in Japan: Epidemiology, diagnosis, treatment. Springer-Verlag Tokyo.

Lim, H., Jung, B. K., Cho, J., Yooyen, T., Shin, E. H., & Chai, J. Y. (2015). Molecular diagnosis of cause of anisakiasis in humans, South Korea. Emerging infectious diseases, 21(2), 342–344. https://doi.org/10.3201/eid2102.140798.

Mattiucci, S., & Nascetti, G. (2008). Advances and trends in the molecular systematics of anisakid nematodes, with implications for their evolutionary ecology and host-parasite co-evolutionary processes. Advances in parasitology, 66, 47–148. https://doi.org/10.1016/S0065-308X(08)00202-9.

Mattiucci, S., Paoletti, M., Borrini, F., Palumbo, M., Palmieri, R. M., Gomes, V., Casati, A., & Nascetti, G. (2011). First molecular identification of the zoonotic parasite Anisakis pegreffii (Nematoda: Anisakidae) in a paraffin-embedded granuloma taken from a case of human intestinal anisakiasis in Italy. BMC infectious diseases, 11, 82. https://doi.org/10.1186/1471-2334-11-82.

Mattiucci, S., Fazii, P., De Rosa, A., Paoletti, M., Megna, A. S., Glielmo, A., De Angelis, M., Costa, A., Meucci, C., Calvaruso, V., Sorrentini, I., Palma, G., Bruschi, F., & Nascetti, G. (2013). Anisakiasis and gastroallergic reactions associated with Anisakis pegreffii infection, Italy. Emerging infectious diseases, 19(3), 496–499. https://doi.org/10.3201/eid1903.121017.

Mattiucci, S., Cipriani, P., Levsen, A., Paoletti, M., & Nascetti, G. (2018). Molecular Epidemiology of Anisakis and Anisakiasis: An Ecological and Evolutionary Road Map. Advances in parasitology, 99, 93–263. https://doi.org/10.1016/bs.apar.2017.12.001.

Meloni, M., Angelucci, G., Merella, P., Siddi, R., Deiana, C., Orrù, G., & Salati, F. (2011). Molecular characterization of Anisakis larvae from fish caught off Sardinia. The Journal of parasitology, 97(5), 908–914. https://doi.org/10.1645/GE-2742.1.

Mladineo, I., Poljak, V., Martínez-Sernández, V., & Ubeira, F. M. (2014). Anti-Anisakis IgE seroprevalence in the healthy Croatian coastal population and associated risk factors. PLoS neglected tropical diseases, 8(2), e2673. https://doi.org/10.1371/journal.pntd.0002673.

Molina-Fernández, D., Adroher, F. J., & Benítez, R. (2018). A scanning electron microscopy study of Anisakis physeteris molecularly identified: from third stage larvae from fish to fourth stage larvae obtained in vitro. Parasitology research, 117(7), 2095–2103. https://doi.org/10.1007/s00436-018-5896-5.

Nadler, S. A., D'Amelio, S., Dailey, M. D., Paggi, L., Siu, S., & Sakanari, J. A. (2005). Molecular phylogenetics and diagnosis of Anisakis, Pseudoterranova, and Contracaecum from northern Pacific marine mammals. The Journal of parasitology, 91(6), 1413–1429. https://doi.org/10.1645/GE-522R.1.

Ozuni, E., Vodica, A., Castrica, M., Brecchia, G., Curone, G., Agradi, S., Miraglia, D., Menchetti, L., Balzaretti, C. M. & Andoni, E. (2021). Prevalence of Anisakis Larvae in Different Fish Species in Southern Albania: Five-Year Monitoring (2016–2020). Applied Sciences, 11(23), 11528. https://doi.org/10.3390/app112311528.

Tamura, K., Stecher, G., & Kumar, S. (2021). MEGA11: Molecular Evolutionary Genetics Analysis Version 11. Molecular biology and evolution, 38(7), 3022–3027. https://doi.org/10.1093/molbev/msab120.

Umehara, A., Kawakami, Y., Matsui, T., Araki, J., & Uchida, A. (2006). Molecular identification of Anisakis simplex sensu stricto and Anisakis pegreffii (Nematoda: Anisakidae) from fish and cetacean in Japanese waters. Parasitology international, 55(4), 267–271. https://doi.org/10.1016/j.parint.2006.07.001.

Zhu, X., Gasser, R. B., Podolska, M., & Chilton, N. B. (1998). Characterisation of anisakid nematodes with zoonotic potential by nuclear ribosomal DNA sequences. International journal for parasitology, 28(12), 1911–1921. https://doi.org/10.1016/s0020-7519(98)00150-7.

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Published

2026-01-16

How to Cite

Vodica, A., Lalle, M. ., & Santolamazza, F. . (2026). First molecular identification of the zoonotic parasite Anisakis pegreffii (Nematoda: Anisakidae) in Albania. Veterinaria Italiana, 62(1). https://doi.org/10.12834/VetIt.3855.37828.2

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