Comparative Analysis of the Methanogen Microbiota Associated to Pasture and Stall Housing in Kazakh Cattle
DOI:
https://doi.org/10.12834/VetIt.3480.24044.2Keywords:
Archea, Cattle, Kazakhstan, Methane, MicrobiotaAbstract
The microbial community of cattle rumen (archaea) are the key players in methane emissions. Methane pollutes the atmosphere and leads to the loss of feed energy. The aim of this study was to comparatively investigate the cattle microbiota, with a particular focus on archaea, in relation to the type of housing: pasture versus stall. A 16S metabarcoding analysis of the intestinal contents of cattle was carried out. Аlpha - diversity of grazing animals showed to be higher compared to animals in the stall period (p=0.002). Beta - diversity confirmed a difference in methanogens and microbiota between animals kept on pasture and those in a stall. Differential abundance analysis showed that the relative abundance of the Methanobacteriacea family in animals in the pasture period was significantly higher compared to animals in the stall period (FDR p = 0.00122). In conclusion, this study demonstrates that the concentration of methanogens in the fecal contents of animals during pasture period was higher than that in animals during the stall period. We recommend feeding grazing animals with concentrates in the evening in order to mitigate methane emissions.
References
Aguerre, M.J., Wattiaux, M.A., Powell, J.M., Broderick, G.A. & Arndt, C. (2011). Effect of forage-to-concentrate ratio in dairy cow diets on emission of methane, carbon dioxide, and ammonia, lactation performance, and manure excretion. Journal of Dairy Science, 94(6), 3081–3093. https://doi.org/10.3168/jds.2010-4011
Bach, А., López-García, A., González-Recio, O., Elcoso, G., Fàbregas, F., Chaucheyras-Durand, F. & Castex, M. (2018). Changes in the rumen and colon microbiota and effects of live yeast dietary supplementation during the transition from the dry period to lactation of dairy cows. Journal of Dairy Science, 102(7), 6180–6198. https://doi.org/10.3168/jds.2018-16105
Daugaliyeva, A., Daugaliyeva, S., Ashanin, A., Kanatbayev, S., Beltramo, C. & Peletto, S. (2022). Study of cattle microbiota in different regions of Kazakhstan using 16S metabarcoding analysis. Scentific Reports, 12(1), 16410. https://doi.org/10.1038/s41598-022-20732-4
Demeyer, D.L. & Henderickx, H.K. (1967). The Effect of C18 Unsaturated Fatty Acids on Methane Production in Vitro by Mixed Rumen Bacteria. Biochimica et Biophysica Acta-Lipids and Lipid Metabolism, 137(3), 484-497. https://doi.org/10.1016/0005-2760(67)90130-0
Freetly, H.C., Lindholm-Perry, A.K., Hales, K.E., Brown-Brandl, T.M., Kim, M., Myer, P.R. & Wells, J.E. (2015). Methane production and methanogen levels in steers that differ in residual gain. Journal of Animal Science, 93(5), 2375–2381. https://doi.org/10.2527/jas.2014-8721
Hammond, K.J., Jones, A.K., Humphries, D.J., Crompton, L.A. & Reynolds, C.K. (2016). Effects of diet forage source and neutral detergent fiber content on milk production of dairy cattle and methane emissions determined using GreenFeed and respiration chamber techniques. Journal of Dairy Science, 99(10), 7904–7917. https://doi.org/10.3168/jds.2015-10759
Freetly, H.C., Dickey, A., Lindholm-Perry, A.K., Thallman R.M., Keele, J.W., Foote, A.P. & Wells J.E. (2020). Digestive tract microbiota of beef cattle that differed in feed efficiency. Journal of Animal Science, 98(2), 1–16. https://doi.org/10.1093/jas/skaa008
Kelly, W.J., Leahy, S.C., Kamke, J., Soni, P., Koike, S., Mackie, R., Seshadri, R., Cook, G.M., Morales, S.E., Greening, C. & Attwood, G.T. (2019). Occurrence and expression of genes encoding methyl-compound production in rumen bacteria. Animal Microbiome, 1(1), 1-15. https://doi.org/10.1186/s42523-019-0016-0
Lin, M., Schaefer, D.M., Zhao, G.Q. & Meng, Q.X. (2013). Effects of nitrate adaptation by rumen inocula donors and substrate fiber proportion on in vitro nitrate disappearance, methanogenesis, and rumen fermentation acid. Animal, 7(7), 1099–1105. https://doi.org/10.1017/S1751731113000116
Liu, J., Liu, F., Cai, W., Jia, C., Bai, Y., He, Y., Zhu, W., Li, R.W. & Song, J. (2020). Diet-induced changes in bacterial communities in the jejunum and their associations with bile acids in Angus beef cattle. Animal Microbiome, 2(1), 33. https://doi.org/ 10.1186/s42523-020-00051-7
Martinez-Fernandez, G., Denman, S.E., Yang, C., Cheung, J., Mitsumori, M., & McSweeney, C.S. (2016). Methane Inhibition Alters the Microbial Community, Hydrogen Flow, and Fermentation Response in the Rumen of Cattle. Frontiers in microbiology, 7, 1122. https://doi.org/10.3389/fmicb.2016.01122
Matthews, С., Crispie, F., Lewis, E., Reid, M., O'Toole P.W. & Cotter P.D. (2019). The rumen microbiome: a crucial consideration when optimising milk and meat production and nitrogen utilisation efficiency. Gut Microbes, 10(2), 115-132. https://doi.org/10.1080/19490976.2018.1505176
Myera, P.R. (2019). Bovine genome-microbiome interactions: metagenomic frontier for the selection of efficient productivity in cattle systems. mSystems, 4(3), e00103-19. https://doi.org/10.1128/mSystems.00103-19
O’Brien, M., Navarro-Villa, A., Purcell, P.J., Boland, T.M. & O’Kiely, P. (2013). Reducing in vitro rumen methanogenesis for two contrasting diets using a series of inclusion rates of different additives. Animal Production Science, 54(2), 141–157. https://doi.org/10.1071/AN12204
Ross, E.M., Moate, P.J., Marett, L., Cocks, B.G. & Hayes, B.J. (2013). Investigating the effect of two methane-mitigating diets on the rumen microbiome using massively parallel sequencing. Journal of Dairy Science, 96(6), 6030–6046. https://doi.org/10.3168/jds.2013-6766
Shoukun, J., Hongtao, Z, Hui, Y., Arash, A., Haitao, S., Gibson, A., Shengli, L., Zhijun, C. & Yajing, W. (2017). Comparison of rumen bacteria distribution in original rumen digesta rumen liquid and solid fractions in lactating Holstein cows. Journal of Animal Science and Biotechnology, 8, 16. https://doi.org/10.1186/s40104-017-0142-z
Szalanski, M., Kristensen, T., Difford, G., Lassen, J., Buitenhuis, A.J., Pszczola, M. & Løvendahl1, P. (2019). Enteric methane emission from Jersey cows during the spring transition from indoor feeding to grazing. Journal of Dairy Science, 102(7), 6319–6329. https://doi.org/10.3168/jds.2018-15984
Vyas, D., McGinn, S.M., Duval, S.M., Kindermann, M.K. & Beauchemin, K.A. (2016). Optimal dose of 3-nitrooxypropanol for decreasing enteric methane emissions from beef cattle fed high-forage and high-grain diets. Animal Production Science, 58(6), 1049-1055. https://doi.org/10.1071/AN15705
Wallace, R.J., Sasson, G., Gregson, Е., Bani, P., Bayat, A.R., Strozzi, F. & Mizrani, I. (2019). A heritable subset of the core rumen microbiome dictates dairy cow productivity and emissions. Science Advances, 5(7), eaav8391. https://doi.org/10.1126/sciadv.aav8391
Wang T., van Dijk, L., Rijnaarts, I., Hermes, G.D.A., de Roos, N.M., Witteman, B.J.M., de Wit, N.J.W., Govers, C., Smidt, H. & Zoetendal, E.G. (2022). Methanogen levels are significantly associated with fecal microbiota composition and alpha diversity in healthy adults and irritable bowel syndrome patients. Microbiology Spectrum, 10(6), e0165322. https://doi.org/10.1128/spectrum.01653-22
